Characterization and myocarditic capabilities of Coxsackie virus B3 variants in selected mouse strains

C. J. Gauntt, P. T. Gomez, P. S. Duffey, J. A. Grant, D. W. Trent, S. M. Witherspoon, R. E. Paque

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Abstract

Two variants of coxsackievirus B3 (CVB3) were compared with the original myocarditic parent variant (CVB3(m)) for myocarditic properties in several strains of mice. The ts1R variant produced little to no myocarditis in any of the nine mouse strains examined. The ts10R variant and CVB3(m) could be differentiated on the basis of the extent of myocarditis induced in mice of selected H-2b and H-2(k) haplotypes and in the female versus the male responses of two other inbred strains. Virus quantities recovered from the hearts of myocarditic mice did not correlate with the extent of disease. The three variants could not be differentiated on the basis of: (i) rate and extent of adsorption to heart tissue homogenates, (ii) kinetic neutralization rates with antiserum directed against CVB3(m), (iii) 125I labeling of surface regions of polypeptides on purified particles, or (iv) rates of heat inactivation of infectivity at 50°C. These data suggest that differences in pathogenicity cannot be attributed to major alterations in capsid polypeptides. Oligonucleotide fingerprint maps of T1 RNase digests of the genomes of purified particles of the three CVB3 variants showed distinct differences. Thus, the extent of the genomes of purified particles of the three CVB3 variants showed distinct differences. Thus, the extent of myocarditis induced by CVB3 variants in a mouse model is affected by some subtle expression of the genome, presumably not involving capsid polypeptides, as well as by the haplotype and sex of a given mouse host species.

Original languageEnglish (US)
Pages (from-to)598-605
Number of pages8
JournalJournal of Virology
Volume52
Issue number2
StatePublished - 1984

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Enterovirus
mice
myocarditis
Myocarditis
polypeptides
capsid
Capsid
Genome
Haplotypes
Peptides
genome
haplotypes
pathogenicity
heart
Ribonuclease T1
heat inactivation
Dermatoglyphics
oligonucleotides
Oligonucleotides
neutralization

ASJC Scopus subject areas

  • Immunology

Cite this

Gauntt, C. J., Gomez, P. T., Duffey, P. S., Grant, J. A., Trent, D. W., Witherspoon, S. M., & Paque, R. E. (1984). Characterization and myocarditic capabilities of Coxsackie virus B3 variants in selected mouse strains. Journal of Virology, 52(2), 598-605.

Characterization and myocarditic capabilities of Coxsackie virus B3 variants in selected mouse strains. / Gauntt, C. J.; Gomez, P. T.; Duffey, P. S.; Grant, J. A.; Trent, D. W.; Witherspoon, S. M.; Paque, R. E.

In: Journal of Virology, Vol. 52, No. 2, 1984, p. 598-605.

Research output: Contribution to journalArticle

Gauntt, CJ, Gomez, PT, Duffey, PS, Grant, JA, Trent, DW, Witherspoon, SM & Paque, RE 1984, 'Characterization and myocarditic capabilities of Coxsackie virus B3 variants in selected mouse strains', Journal of Virology, vol. 52, no. 2, pp. 598-605.
Gauntt CJ, Gomez PT, Duffey PS, Grant JA, Trent DW, Witherspoon SM et al. Characterization and myocarditic capabilities of Coxsackie virus B3 variants in selected mouse strains. Journal of Virology. 1984;52(2):598-605.
Gauntt, C. J. ; Gomez, P. T. ; Duffey, P. S. ; Grant, J. A. ; Trent, D. W. ; Witherspoon, S. M. ; Paque, R. E. / Characterization and myocarditic capabilities of Coxsackie virus B3 variants in selected mouse strains. In: Journal of Virology. 1984 ; Vol. 52, No. 2. pp. 598-605.
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