@article{43a66192b28a4941b9c2629dcc34a478,
title = "Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates",
abstract = "Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, with robust excretion of ZIKV RNA in urine, saliva, and lacrimal fluid. Necropsy of two infected animals revealed that systematic infections involving central nervous system and visceral organs were established at the acute phrase. ZIKV initially targeted the intestinal tracts, spleen, and parotid glands, and retained in spleen and lymph nodes till 10 days post infection. ZIKV-specific immune responses were readily induced in all inoculated animals. The non-human primate model described here provides a valuable platform to study ZIKV pathogenesis and to evaluate vaccine and therapeutics.",
keywords = "Lacrimal fluid, Non-human primate model, Target organ, Zika virus",
author = "Li, {Xiao Feng} and Dong, {Hao Long} and Huang, {Xing Yao} and Qiu, {Ye Feng} and Wang, {Hong Jiang} and Deng, {Yong Qiang} and Zhang, {Na Na} and Qing Ye and Hui Zhao and Liu, {Zhong Yu} and Hang Fan and An, {Xiao Ping} and Sun, {Shi Hui} and Bo Gao and Fa, {Yun Zhi} and Tong, {Yi Gang} and Zhang, {Fu Chun} and Gao, {George F.} and Cao, {Wu Chun} and Shi, {Pei Yong} and Qin, {Cheng Feng}",
note = "Funding Information: We thank the veterinarians from Laboratory Animal Center, Academy of Military Medical Science, for their excellent technical support; and Drs. Jing An, Zhiheng Xu, Xia Jia, and Bo Zhang for helpful discussion. This work was supported by the State Key Laboratory of Pathogen and Biosecurity (no. SKLPBS1601 ), the Guangzhou Science and Technology Program for Public Wellbeing (no. 201508020263 , and no. 2014Y2-00550 ), the Beijing Nova Program (no. 2016110 , and no. 2010B041 ), and the National Key Research and Development Project of China (no. 2016YFD0500304 ). CFQ was supported by the Excellent Young Scientist Program from the NSFC of China (no. 81522025 ) and the Newton Advanced Fellowship from the Academy of Medical Sciences , UK and the NSFC of China (No. 81661130162 ). PYS was partially supported by NIH grant AI087856 , and a grant from Pan-American Health Organization and World Health Organization . All authors declared no conflicts of interest. Publisher Copyright: {\textcopyright} 2016 The Authors",
year = "2016",
month = oct,
day = "1",
doi = "10.1016/j.ebiom.2016.09.022",
language = "English (US)",
volume = "12",
pages = "170--177",
journal = "EBioMedicine",
issn = "2352-3964",
publisher = "Elsevier BV",
}