Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates

Xiao Feng Li; Hao Long Dong; Xing Yao Huang; Ye Feng Qiu; Hong Jiang Wang; Yong Qiang Deng; Na Na Zhang; Qing Ye; Hui Zhao; Zhong Yu Liu; Hang Fan; Xiao Ping An; Shi Hui Sun; Bo Gao; Yun Zhi Fa; Yi Gang Tong; Fu Chun Zhang; George F. Gao; Wu Chun Cao; Pei Yong Shi; Cheng Feng Qin

doi:10.1016/j.ebiom.2016.09.022

Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates

Xiao Feng Li, Hao Long Dong, Xing Yao Huang, Ye Feng Qiu, Hong Jiang Wang, Yong Qiang Deng, Na Na Zhang, Qing Ye, Hui Zhao, Zhong Yu Liu, Hang Fan, Xiao Ping An, Shi Hui Sun, Bo Gao, Yun Zhi Fa, Yi Gang Tong, Fu Chun Zhang, George F. Gao, Wu Chun Cao, Pei Yong ShiCheng Feng Qin

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

103 Scopus citations

Abstract

Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, with robust excretion of ZIKV RNA in urine, saliva, and lacrimal fluid. Necropsy of two infected animals revealed that systematic infections involving central nervous system and visceral organs were established at the acute phrase. ZIKV initially targeted the intestinal tracts, spleen, and parotid glands, and retained in spleen and lymph nodes till 10 days post infection. ZIKV-specific immune responses were readily induced in all inoculated animals. The non-human primate model described here provides a valuable platform to study ZIKV pathogenesis and to evaluate vaccine and therapeutics.

Original language	English (US)
Pages (from-to)	170-177
Number of pages	8
Journal	EBioMedicine
Volume	12
DOIs	https://doi.org/10.1016/j.ebiom.2016.09.022
State	Published - Oct 1 2016

Keywords

Lacrimal fluid
Non-human primate model
Target organ
Zika virus

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.ebiom.2016.09.022

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Li, X. F., Dong, H. L., Huang, X. Y., Qiu, Y. F., Wang, H. J., Deng, Y. Q., Zhang, N. N., Ye, Q., Zhao, H., Liu, Z. Y., Fan, H., An, X. P., Sun, S. H., Gao, B., Fa, Y. Z., Tong, Y. G., Zhang, F. C., Gao, G. F., Cao, W. C., ... Qin, C. F. (2016). Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates. EBioMedicine, 12, 170-177. https://doi.org/10.1016/j.ebiom.2016.09.022

@article{43a66192b28a4941b9c2629dcc34a478,

title = "Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates",

abstract = "Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, with robust excretion of ZIKV RNA in urine, saliva, and lacrimal fluid. Necropsy of two infected animals revealed that systematic infections involving central nervous system and visceral organs were established at the acute phrase. ZIKV initially targeted the intestinal tracts, spleen, and parotid glands, and retained in spleen and lymph nodes till 10 days post infection. ZIKV-specific immune responses were readily induced in all inoculated animals. The non-human primate model described here provides a valuable platform to study ZIKV pathogenesis and to evaluate vaccine and therapeutics.",

keywords = "Lacrimal fluid, Non-human primate model, Target organ, Zika virus",

author = "Li, {Xiao Feng} and Dong, {Hao Long} and Huang, {Xing Yao} and Qiu, {Ye Feng} and Wang, {Hong Jiang} and Deng, {Yong Qiang} and Zhang, {Na Na} and Qing Ye and Hui Zhao and Liu, {Zhong Yu} and Hang Fan and An, {Xiao Ping} and Sun, {Shi Hui} and Bo Gao and Fa, {Yun Zhi} and Tong, {Yi Gang} and Zhang, {Fu Chun} and Gao, {George F.} and Cao, {Wu Chun} and Shi, {Pei Yong} and Qin, {Cheng Feng}",

note = "Funding Information: We thank the veterinarians from Laboratory Animal Center, Academy of Military Medical Science, for their excellent technical support; and Drs. Jing An, Zhiheng Xu, Xia Jia, and Bo Zhang for helpful discussion. This work was supported by the State Key Laboratory of Pathogen and Biosecurity (no. SKLPBS1601 ), the Guangzhou Science and Technology Program for Public Wellbeing (no. 201508020263 , and no. 2014Y2-00550 ), the Beijing Nova Program (no. 2016110 , and no. 2010B041 ), and the National Key Research and Development Project of China (no. 2016YFD0500304 ). CFQ was supported by the Excellent Young Scientist Program from the NSFC of China (no. 81522025 ) and the Newton Advanced Fellowship from the Academy of Medical Sciences , UK and the NSFC of China (No. 81661130162 ). PYS was partially supported by NIH grant AI087856 , and a grant from Pan-American Health Organization and World Health Organization . All authors declared no conflicts of interest. Publisher Copyright: {\textcopyright} 2016 The Authors",

year = "2016",

month = oct,

day = "1",

doi = "10.1016/j.ebiom.2016.09.022",

language = "English (US)",

volume = "12",

pages = "170--177",

journal = "EBioMedicine",

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T1 - Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates

AU - Li, Xiao Feng

AU - Dong, Hao Long

AU - Huang, Xing Yao

AU - Qiu, Ye Feng

AU - Wang, Hong Jiang

AU - Deng, Yong Qiang

AU - Zhang, Na Na

AU - Ye, Qing

AU - Zhao, Hui

AU - Liu, Zhong Yu

AU - Fan, Hang

AU - An, Xiao Ping

AU - Sun, Shi Hui

AU - Gao, Bo

AU - Fa, Yun Zhi

AU - Tong, Yi Gang

AU - Zhang, Fu Chun

AU - Gao, George F.

AU - Cao, Wu Chun

AU - Shi, Pei Yong

AU - Qin, Cheng Feng

N1 - Funding Information: We thank the veterinarians from Laboratory Animal Center, Academy of Military Medical Science, for their excellent technical support; and Drs. Jing An, Zhiheng Xu, Xia Jia, and Bo Zhang for helpful discussion. This work was supported by the State Key Laboratory of Pathogen and Biosecurity (no. SKLPBS1601 ), the Guangzhou Science and Technology Program for Public Wellbeing (no. 201508020263 , and no. 2014Y2-00550 ), the Beijing Nova Program (no. 2016110 , and no. 2010B041 ), and the National Key Research and Development Project of China (no. 2016YFD0500304 ). CFQ was supported by the Excellent Young Scientist Program from the NSFC of China (no. 81522025 ) and the Newton Advanced Fellowship from the Academy of Medical Sciences , UK and the NSFC of China (No. 81661130162 ). PYS was partially supported by NIH grant AI087856 , and a grant from Pan-American Health Organization and World Health Organization . All authors declared no conflicts of interest. Publisher Copyright: © 2016 The Authors

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, with robust excretion of ZIKV RNA in urine, saliva, and lacrimal fluid. Necropsy of two infected animals revealed that systematic infections involving central nervous system and visceral organs were established at the acute phrase. ZIKV initially targeted the intestinal tracts, spleen, and parotid glands, and retained in spleen and lymph nodes till 10 days post infection. ZIKV-specific immune responses were readily induced in all inoculated animals. The non-human primate model described here provides a valuable platform to study ZIKV pathogenesis and to evaluate vaccine and therapeutics.

AB - Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, with robust excretion of ZIKV RNA in urine, saliva, and lacrimal fluid. Necropsy of two infected animals revealed that systematic infections involving central nervous system and visceral organs were established at the acute phrase. ZIKV initially targeted the intestinal tracts, spleen, and parotid glands, and retained in spleen and lymph nodes till 10 days post infection. ZIKV-specific immune responses were readily induced in all inoculated animals. The non-human primate model described here provides a valuable platform to study ZIKV pathogenesis and to evaluate vaccine and therapeutics.

KW - Lacrimal fluid

KW - Non-human primate model

KW - Target organ

KW - Zika virus

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M3 - Article

C2 - 27693104

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SN - 2352-3964

VL - 12

SP - 170

EP - 177

JO - EBioMedicine

JF - EBioMedicine

ER -

Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates

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Keywords

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