Characterization of a cocaine binding protein in human placenta

Mahmoud Ahmed, De He Zhou, Dev Maulik, Mohyee E. Eldefrawi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

[3H]-Cocaine binding sites are identified in human placental villus tissue plasma membranes. These binding sites are associated with a protein and show saturable and specific binding of [3H]-cocaine with a high affinity site of 170 fmole/mg protein (Kd 16.7 nM). The binding is lost with pretreatment with trypsin or heat. The membrane bound protein is solubilized with the detergent 3-(3-cholamidopropyl)dimethyl-ammonio-1-propane sulphonate (CHAPS) with retention of its saturable and specific binding of [3H]-cocaine. The detergent-protein complex migrates on a sepharose CL-6B gel chromatography column as a protein with an apparent molecular weight of 75,900. The protein has an S20,w value of 5.1. The binding of this protein to norcocaine, pseudococaine, nomifensine, imipramine, desipramine, amphetamine and dopamine indicates that it shares some, but not all, the properties of the brain cocaine receptor. The physiologic significance of this protein in human placenta is currently unclear.

Original languageEnglish (US)
Pages (from-to)553-561
Number of pages9
JournalLife Sciences
Volume46
Issue number8
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Cocaine
Placenta
Carrier Proteins
Proteins
Detergents
Binding Sites
Nomifensine
Chorionic Villi
Propane
Desipramine
Imipramine
Amphetamine
Column chromatography
Trypsin
Cell membranes
Gel Chromatography
Dopamine
Membrane Proteins
Hot Temperature
Molecular Weight

ASJC Scopus subject areas

  • Pharmacology

Cite this

Characterization of a cocaine binding protein in human placenta. / Ahmed, Mahmoud; Zhou, De He; Maulik, Dev; Eldefrawi, Mohyee E.

In: Life Sciences, Vol. 46, No. 8, 1990, p. 553-561.

Research output: Contribution to journalArticle

Ahmed, Mahmoud ; Zhou, De He ; Maulik, Dev ; Eldefrawi, Mohyee E. / Characterization of a cocaine binding protein in human placenta. In: Life Sciences. 1990 ; Vol. 46, No. 8. pp. 553-561.
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