Characterization of a dengue type-specific epitope on dengue 3 virus envelope protein domain III

Kiyohiko Matsui, Gregory D. Gromowski, Li Li, Alan Barrett

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Dengue virus (DENV) is a mosquito-borne disease caused by four genetically and serologically related viruses termed DENV-1, -2, -3 and -4. The DENV envelope (E) protein ectodomain can be divided into three structural domains designated ED1, ED2 and ED3. The ED3 domain contains DENV type-specific and DENV complex-reactive antigenic sites. To date, nearly all antigenic studies on the E protein have focused on DENV-2. In this study, the epitope recognized by a DENV-3 type-specific monoclonal antibody (mAb 14A4-8) was mapped to the DENV-3 ED3 domain using a combination of physical and biological techniques. Epitope mapping revealed that amino acid residues V305, L306, K308, E309, V310, K325, A329, G381 and I387 were critical for the binding of mAb 14A4-8 and amino acid residues T303, K307, K386, W389 and R391 were peripheral residues for this epitope. The location of the mAb 14A4-8 epitope overlaps with the DENV complex-reactive antigenic site in the DENV-3 ED3 domain.

Original languageEnglish (US)
Pages (from-to)2249-2253
Number of pages5
JournalJournal of General Virology
Volume91
Issue number9
DOIs
StatePublished - Sep 2010

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Viral Envelope Proteins
Dengue Virus
Dengue
Epitopes
Catalytic Domain
Protein Domains
Epitope Mapping
Amino Acids
Culicidae
Monoclonal Antibodies

ASJC Scopus subject areas

  • Virology

Cite this

Characterization of a dengue type-specific epitope on dengue 3 virus envelope protein domain III. / Matsui, Kiyohiko; Gromowski, Gregory D.; Li, Li; Barrett, Alan.

In: Journal of General Virology, Vol. 91, No. 9, 09.2010, p. 2249-2253.

Research output: Contribution to journalArticle

Matsui, Kiyohiko ; Gromowski, Gregory D. ; Li, Li ; Barrett, Alan. / Characterization of a dengue type-specific epitope on dengue 3 virus envelope protein domain III. In: Journal of General Virology. 2010 ; Vol. 91, No. 9. pp. 2249-2253.
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