Abstract
Yersinia pestis is a Gram-negative bacterium, and the causative agent of bubonic plague and pneumonic plague. Because of its potential use as a biological warfare weapon, the plague bacterium has been placed on the list of category A select agents. The dynamics of pneumonic infection following aerosolization of the highly virulent Y. pestis CO92 strain have been poorly studied; therefore, the purpose of this study was to determine the LD50 dose, bacterial dissemination, cytokine/ chemokine production and tissue damage in Swiss-Webster mice over a 72 h course of infection. We exposed mice in a whole-body Madison chamber to various doses of Y. pestis CO92 aerosolized by a Collison nebulizer, and determined that the LD50 presented dose (Dp) of the bacterium in the lungs was 2.1 × 103 c.f.u. In a subsequent study, we infected mice at a Dp of 1.3 × 104 c.f.u., and harvested organs and blood at 1, 24, 48 and 72 h post-infection. Histopathological examination, in addition to measurement of bacterial dissemination and cytokine/chemokine analysis, indicated progressive tissue injury, and an increased number of animals succumbing to infection over the course of the experiment. Using these data, we were able to characterize the mouse plague model following aerosolization of Y. pestis CO92.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1939-1948 |
| Number of pages | 10 |
| Journal | Microbiology |
| Volume | 154 |
| Issue number | 7 |
| DOIs | |
| State | Published - 2008 |
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- Microbiology
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Characterization of a mouse model of plague after aerosolization of Yersinia pestis CO92. / Agar, Stacy L.; Sha, Jian; Foltz, Sheri M.; Erova, Tatiana E.; Walberg, Kristin G.; Parham, Todd E.; Baze, Wallace B.; Suarez, Giovanni; Peterson, Johnny; Chopra, Ashok.
In: Microbiology, Vol. 154, No. 7, 2008, p. 1939-1948.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of a mouse model of plague after aerosolization of Yersinia pestis CO92
AU - Agar, Stacy L.
AU - Sha, Jian
AU - Foltz, Sheri M.
AU - Erova, Tatiana E.
AU - Walberg, Kristin G.
AU - Parham, Todd E.
AU - Baze, Wallace B.
AU - Suarez, Giovanni
AU - Peterson, Johnny
AU - Chopra, Ashok
PY - 2008
Y1 - 2008
N2 - Yersinia pestis is a Gram-negative bacterium, and the causative agent of bubonic plague and pneumonic plague. Because of its potential use as a biological warfare weapon, the plague bacterium has been placed on the list of category A select agents. The dynamics of pneumonic infection following aerosolization of the highly virulent Y. pestis CO92 strain have been poorly studied; therefore, the purpose of this study was to determine the LD50 dose, bacterial dissemination, cytokine/ chemokine production and tissue damage in Swiss-Webster mice over a 72 h course of infection. We exposed mice in a whole-body Madison chamber to various doses of Y. pestis CO92 aerosolized by a Collison nebulizer, and determined that the LD50 presented dose (Dp) of the bacterium in the lungs was 2.1 × 103 c.f.u. In a subsequent study, we infected mice at a Dp of 1.3 × 104 c.f.u., and harvested organs and blood at 1, 24, 48 and 72 h post-infection. Histopathological examination, in addition to measurement of bacterial dissemination and cytokine/chemokine analysis, indicated progressive tissue injury, and an increased number of animals succumbing to infection over the course of the experiment. Using these data, we were able to characterize the mouse plague model following aerosolization of Y. pestis CO92.
AB - Yersinia pestis is a Gram-negative bacterium, and the causative agent of bubonic plague and pneumonic plague. Because of its potential use as a biological warfare weapon, the plague bacterium has been placed on the list of category A select agents. The dynamics of pneumonic infection following aerosolization of the highly virulent Y. pestis CO92 strain have been poorly studied; therefore, the purpose of this study was to determine the LD50 dose, bacterial dissemination, cytokine/ chemokine production and tissue damage in Swiss-Webster mice over a 72 h course of infection. We exposed mice in a whole-body Madison chamber to various doses of Y. pestis CO92 aerosolized by a Collison nebulizer, and determined that the LD50 presented dose (Dp) of the bacterium in the lungs was 2.1 × 103 c.f.u. In a subsequent study, we infected mice at a Dp of 1.3 × 104 c.f.u., and harvested organs and blood at 1, 24, 48 and 72 h post-infection. Histopathological examination, in addition to measurement of bacterial dissemination and cytokine/chemokine analysis, indicated progressive tissue injury, and an increased number of animals succumbing to infection over the course of the experiment. Using these data, we were able to characterize the mouse plague model following aerosolization of Y. pestis CO92.
UR - http://www.scopus.com/inward/record.url?scp=51149099737&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51149099737&partnerID=8YFLogxK
U2 - 10.1099/mic.0.2008/017335-0
DO - 10.1099/mic.0.2008/017335-0
M3 - Article
VL - 154
SP - 1939
EP - 1948
JO - Microbiology (United Kingdom)
JF - Microbiology (United Kingdom)
SN - 1350-0872
IS - 7
ER -