Characterization of a murine model of fetal programming of atherosclerosis

Nima Goharkhay, Elena Sbrana, Phyllis K. Gamble, Esther H. Tamayo, Ancizar Betancourt, Karina Villarreal, Gary D.V. Hankins, George R. Saade, Monica Longo

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Objective: The objective of the study was to investigate the effect of fetal programming on the development of atherosclerosis in the offspring in a mouse model. Study Design: Male and female mice of the wild type and the knockout for the apoprotein E (apoE) gene were cross-bred to obtain all 4 possible genetic offspring types. The offspring were kept on regular chow and killed at 8 months of age. Levels of total cholesterol and triglycerides were determined. The aortic arch was examined for the presence and severity of atherosclerosis. Kidney and liver sections were analyzed for pathologic changes. Results: We found increased total cholesterol levels and incidence of atherosclerosis in offspring born to hypercholesterolemic mothers as compared with genomically similar animals born to wild-type mothers. These animals also showed kidney and liver lesions consistent with chronic hypercholesterolemia. Conclusions: There is a strong effect of fetal programming on the development of atherosclerosis in the apoE mouse model.

Original languageEnglish (US)
Pages (from-to)416.e1-416.e5
JournalAmerican journal of obstetrics and gynecology
Volume197
Issue number4
DOIs
StatePublished - Oct 2007

Keywords

  • atherosclerosis
  • fetal development
  • fetal programming
  • hypercholesterolemia
  • mouse model

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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