Characterization of a novel 8-oxoguanine-DNA glycosylase activity in Escherichia coli and identification of the enzyme as endonuclease VIII

Tapas Hazra, Tadahide Izumi, Radhika Venkataraman, Yoke W. Kow, Miral Dizdaroglu, Sankar Mitra

Research output: Contribution to journalArticle

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Abstract

8-Oxoguanine (G*), induced by reactive oxygen species, is mutagenic because it mispairs with A. The major G*-DNA glycosylase (OGG), namely, OGG1 in eukaryotes, or MutM in Escherichia coli, excises G* when paired in DNA with C, G, and T, but not A, presumably because removal of G* from a G*·A pair would be mutagenic. However, repair of G* will prevent mutation when it is incorporated in the nascent strand opposite A. This could be carried out by a second OGG, OGG2, identified in yeast and human cells. We have characterized a new OGG activity in E. coli and then identified it to be endonuclease VIII (Nei), discovered as a damaged pyrimidine-specific DNA glycosylase. Nei shares sequence homology and reaction mechanism with MutM and is similar to human OGG2 in being able to excise G* when paired with A (or G). Kinetic analysis of wild type Nei showed that it has significant activity for excising G* relative to dihydrouracil. The presence of OGG2 type enzyme in both E. coli and eukaryotes, which is at least as efficient in excising G* from a G*·A (or G) pair as from a G*·C pair, supports the possibility of G* repair in the nascent DNA strand.

Original languageEnglish (US)
Pages (from-to)27762-27767
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number36
DOIs
StatePublished - Sep 8 2000

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Deoxyribonuclease (Pyrimidine Dimer)
DNA Glycosylases
Escherichia coli
Eukaryota
Repair
Enzymes
DNA
Sequence Homology
Yeast
Reactive Oxygen Species
Yeasts
Cells
Mutation
Kinetics
8-hydroxyguanine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of a novel 8-oxoguanine-DNA glycosylase activity in Escherichia coli and identification of the enzyme as endonuclease VIII. / Hazra, Tapas; Izumi, Tadahide; Venkataraman, Radhika; Kow, Yoke W.; Dizdaroglu, Miral; Mitra, Sankar.

In: Journal of Biological Chemistry, Vol. 275, No. 36, 08.09.2000, p. 27762-27767.

Research output: Contribution to journalArticle

Hazra, Tapas ; Izumi, Tadahide ; Venkataraman, Radhika ; Kow, Yoke W. ; Dizdaroglu, Miral ; Mitra, Sankar. / Characterization of a novel 8-oxoguanine-DNA glycosylase activity in Escherichia coli and identification of the enzyme as endonuclease VIII. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 36. pp. 27762-27767.
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