Characterization of a novel megalocytivirus isolated from european chub (Squalius cephalus)

Maya A. Halaly, Kuttichantran Subramaniam, Samantha A. Koda, Vsevolod Popov, David Stone, Keith Way, Thomas B. Waltzek

Research output: Contribution to journalArticle

Abstract

A novel virus from moribund European chub (Squalius cephalus) was isolated on epithelioma papulosum cyprini (EPC) cells. Transmission electron microscopic examination revealed abundant non-enveloped, hexagonal virus particles in the cytoplasm of infected EPC cells consistent with an iridovirus. Illumina MiSeq sequence data enabled the assembly and annotation of the full genome (128,216 bp encoding 108 open reading frames) of the suspected iridovirus. Maximum Likelihood phylogenetic analyses based on 25 iridovirus core genes supported the European chub iridovirus (ECIV) as being the sister species to the recently-discovered scale drop disease virus (SDDV), which together form the most basal megalocytivirus clade. Genetic analyses of the ECIV major capsid protein and ATPase genes revealed the greatest nucleotide identity to members of the genus Megalocytivirus including SDDV. These data support ECIV as a novel member within the genus Megalocytivirus. Experimental challenge studies are needed to fulfill River’s postulates and determine whether ECIV induces the pathognomonic microscopic lesions (i.e., megalocytes with basophilic cytoplasmic inclusions) observed in megalocytivirus infections.

Original languageEnglish (US)
Article number440
JournalViruses
Volume11
Issue number5
DOIs
StatePublished - May 1 2019

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Iridoviridae
Iridovirus
Cyprinidae
Virus Diseases
Carcinoma
Inclusion Bodies
Rivers
Virion
Open Reading Frames
Genes
Adenosine Triphosphatases
Cytoplasm
Nucleotides
Genome
Electrons
Viruses

Keywords

  • European chub
  • Iridovirus
  • Megalocytivirus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Halaly, M. A., Subramaniam, K., Koda, S. A., Popov, V., Stone, D., Way, K., & Waltzek, T. B. (2019). Characterization of a novel megalocytivirus isolated from european chub (Squalius cephalus). Viruses, 11(5), [440]. https://doi.org/10.3390/v11050440

Characterization of a novel megalocytivirus isolated from european chub (Squalius cephalus). / Halaly, Maya A.; Subramaniam, Kuttichantran; Koda, Samantha A.; Popov, Vsevolod; Stone, David; Way, Keith; Waltzek, Thomas B.

In: Viruses, Vol. 11, No. 5, 440, 01.05.2019.

Research output: Contribution to journalArticle

Halaly, MA, Subramaniam, K, Koda, SA, Popov, V, Stone, D, Way, K & Waltzek, TB 2019, 'Characterization of a novel megalocytivirus isolated from european chub (Squalius cephalus)', Viruses, vol. 11, no. 5, 440. https://doi.org/10.3390/v11050440
Halaly, Maya A. ; Subramaniam, Kuttichantran ; Koda, Samantha A. ; Popov, Vsevolod ; Stone, David ; Way, Keith ; Waltzek, Thomas B. / Characterization of a novel megalocytivirus isolated from european chub (Squalius cephalus). In: Viruses. 2019 ; Vol. 11, No. 5.
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