Characterization of a novel Negevirus and a novel Bunyavirus isolated from Culex (Culex) declarator mosquitoes in Trinidad

Albert J. Auguste, Christine V F Carrington, Naomi L. Forrester, Vsevolod Popov, Hilda Guzman, Steven Widen, Thomas Wood, Scott Weaver, Robert B. Tesh

Research output: Contribution to journalArticle

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Abstract

Pools of mosquitoes were tested for insect-specific viruses using cytopathic effect (CPE) assays on Aedes albopictus (C6/36) cells. Illumina sequencing of RNA from pool TR7094, which produced extensive CPE 2 days post-infection, yielded the complete genome sequences of a previously unknown Bunyavirus, designated Cumuto virus (CUMV), and a second virus designated Wallerfield virus (WALV). WALV shared highest amino acid identity (60.1%) with Dezidougou virus from Côte d'Ivoire, a positive-sense, single-strand RNA, insect-specific virus belonging to the newly proposed genus Negevirus associated with mosquitoes and phlebotomine sandflies. The S, M and L segments of CUMV were most closely related to those of Gouleako virus, also from Côte d'Ivoire (amino acid identities of 36 %, 38% and 54% respectively). Neither virus produced CPE on vertebrate cells, or illness in newborn mice. Isolation and characterization of these viruses increase our knowledge of the geographical distribution, diversity and host range ofmosquito-specific bunyaviruses and negeviruses.

Original languageEnglish (US)
Pages (from-to)481-485
Number of pages5
JournalJournal of General Virology
Volume95
Issue numberPART 2
DOIs
StatePublished - Feb 2014

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Orthobunyavirus
Trinidad and Tobago
Culex
Culicidae
Viruses
Insect Viruses
RNA Sequence Analysis
Amino Acids
Psychodidae
Aedes
Host Specificity
Vertebrates
Genome
RNA

ASJC Scopus subject areas

  • Virology

Cite this

Characterization of a novel Negevirus and a novel Bunyavirus isolated from Culex (Culex) declarator mosquitoes in Trinidad. / Auguste, Albert J.; Carrington, Christine V F; Forrester, Naomi L.; Popov, Vsevolod; Guzman, Hilda; Widen, Steven; Wood, Thomas; Weaver, Scott; Tesh, Robert B.

In: Journal of General Virology, Vol. 95, No. PART 2, 02.2014, p. 481-485.

Research output: Contribution to journalArticle

Auguste, Albert J. ; Carrington, Christine V F ; Forrester, Naomi L. ; Popov, Vsevolod ; Guzman, Hilda ; Widen, Steven ; Wood, Thomas ; Weaver, Scott ; Tesh, Robert B. / Characterization of a novel Negevirus and a novel Bunyavirus isolated from Culex (Culex) declarator mosquitoes in Trinidad. In: Journal of General Virology. 2014 ; Vol. 95, No. PART 2. pp. 481-485.
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