Characterization of a novel species of adenovirus from Japanese microbat and role of CXADR as its entry factor

Tomoya Kobayashi, Hiromichi Matsugo, Junki Maruyama, Haruhiko Kamiki, Ayato Takada, Ken Maeda, Akiko Takenaka-Uema, Yukinobu Tohya, Shin Murakami, Taisuke Horimoto

Research output: Contribution to journalArticle

Abstract

Recently, bat adenoviruses (BtAdVs) of genus Mastadenovirus have been isolated from various bat species, some of them displaying a wide host range in cell culture. In this study, we isolated two BtAdVs from Japanese wild microbats. While one isolate was classified as Bat mastadenovirus A, the other was phylogenetically independent of other BtAdVs. It was rather related to, but serologically different from, canine adenoviruses. We propose that the latter, isolated from Asian parti-colored bat, should be assigned to a novel species of Bat mastadenovirus. Both isolates replicated in various mammalian cell lines, implying their wide cell tropism. To gain insight into cell tropism of these BtAdVs, we investigated the coxsackievirus and adenovirus receptor (CXADR) for virus entry to the cells. We prepared CXADR-knockout canine kidney cells and found that replication of BtAdVs was significantly hampered in these cells. For confirmation, their replication in canine CXADR-addback cells was rescued to the levels with the original cells. We also found that viral replication was corrected in human or bat CXADR-transduced cells to similar levels as in canine CXADR-addback cells. These results suggest that BtAdVs were able to use several mammalian-derived CXADRs as entry factors.

Original languageEnglish (US)
Article number573
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019
Externally publishedYes

Fingerprint

Enterovirus
Adenoviridae
Mastadenovirus
Canine Adenoviruses
Tropism
adenovirus receptor
Virus Internalization
Host Specificity
Canidae
Cell Culture Techniques
Kidney
Cell Line

ASJC Scopus subject areas

  • General

Cite this

Characterization of a novel species of adenovirus from Japanese microbat and role of CXADR as its entry factor. / Kobayashi, Tomoya; Matsugo, Hiromichi; Maruyama, Junki; Kamiki, Haruhiko; Takada, Ayato; Maeda, Ken; Takenaka-Uema, Akiko; Tohya, Yukinobu; Murakami, Shin; Horimoto, Taisuke.

In: Scientific Reports, Vol. 9, No. 1, 573, 01.12.2019.

Research output: Contribution to journalArticle

Kobayashi, T, Matsugo, H, Maruyama, J, Kamiki, H, Takada, A, Maeda, K, Takenaka-Uema, A, Tohya, Y, Murakami, S & Horimoto, T 2019, 'Characterization of a novel species of adenovirus from Japanese microbat and role of CXADR as its entry factor', Scientific Reports, vol. 9, no. 1, 573. https://doi.org/10.1038/s41598-018-37224-z
Kobayashi, Tomoya ; Matsugo, Hiromichi ; Maruyama, Junki ; Kamiki, Haruhiko ; Takada, Ayato ; Maeda, Ken ; Takenaka-Uema, Akiko ; Tohya, Yukinobu ; Murakami, Shin ; Horimoto, Taisuke. / Characterization of a novel species of adenovirus from Japanese microbat and role of CXADR as its entry factor. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
@article{e92898b819904e15abe56ca8f3b9fa77,
title = "Characterization of a novel species of adenovirus from Japanese microbat and role of CXADR as its entry factor",
abstract = "Recently, bat adenoviruses (BtAdVs) of genus Mastadenovirus have been isolated from various bat species, some of them displaying a wide host range in cell culture. In this study, we isolated two BtAdVs from Japanese wild microbats. While one isolate was classified as Bat mastadenovirus A, the other was phylogenetically independent of other BtAdVs. It was rather related to, but serologically different from, canine adenoviruses. We propose that the latter, isolated from Asian parti-colored bat, should be assigned to a novel species of Bat mastadenovirus. Both isolates replicated in various mammalian cell lines, implying their wide cell tropism. To gain insight into cell tropism of these BtAdVs, we investigated the coxsackievirus and adenovirus receptor (CXADR) for virus entry to the cells. We prepared CXADR-knockout canine kidney cells and found that replication of BtAdVs was significantly hampered in these cells. For confirmation, their replication in canine CXADR-addback cells was rescued to the levels with the original cells. We also found that viral replication was corrected in human or bat CXADR-transduced cells to similar levels as in canine CXADR-addback cells. These results suggest that BtAdVs were able to use several mammalian-derived CXADRs as entry factors.",
author = "Tomoya Kobayashi and Hiromichi Matsugo and Junki Maruyama and Haruhiko Kamiki and Ayato Takada and Ken Maeda and Akiko Takenaka-Uema and Yukinobu Tohya and Shin Murakami and Taisuke Horimoto",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-018-37224-z",
language = "English (US)",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Characterization of a novel species of adenovirus from Japanese microbat and role of CXADR as its entry factor

AU - Kobayashi, Tomoya

AU - Matsugo, Hiromichi

AU - Maruyama, Junki

AU - Kamiki, Haruhiko

AU - Takada, Ayato

AU - Maeda, Ken

AU - Takenaka-Uema, Akiko

AU - Tohya, Yukinobu

AU - Murakami, Shin

AU - Horimoto, Taisuke

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Recently, bat adenoviruses (BtAdVs) of genus Mastadenovirus have been isolated from various bat species, some of them displaying a wide host range in cell culture. In this study, we isolated two BtAdVs from Japanese wild microbats. While one isolate was classified as Bat mastadenovirus A, the other was phylogenetically independent of other BtAdVs. It was rather related to, but serologically different from, canine adenoviruses. We propose that the latter, isolated from Asian parti-colored bat, should be assigned to a novel species of Bat mastadenovirus. Both isolates replicated in various mammalian cell lines, implying their wide cell tropism. To gain insight into cell tropism of these BtAdVs, we investigated the coxsackievirus and adenovirus receptor (CXADR) for virus entry to the cells. We prepared CXADR-knockout canine kidney cells and found that replication of BtAdVs was significantly hampered in these cells. For confirmation, their replication in canine CXADR-addback cells was rescued to the levels with the original cells. We also found that viral replication was corrected in human or bat CXADR-transduced cells to similar levels as in canine CXADR-addback cells. These results suggest that BtAdVs were able to use several mammalian-derived CXADRs as entry factors.

AB - Recently, bat adenoviruses (BtAdVs) of genus Mastadenovirus have been isolated from various bat species, some of them displaying a wide host range in cell culture. In this study, we isolated two BtAdVs from Japanese wild microbats. While one isolate was classified as Bat mastadenovirus A, the other was phylogenetically independent of other BtAdVs. It was rather related to, but serologically different from, canine adenoviruses. We propose that the latter, isolated from Asian parti-colored bat, should be assigned to a novel species of Bat mastadenovirus. Both isolates replicated in various mammalian cell lines, implying their wide cell tropism. To gain insight into cell tropism of these BtAdVs, we investigated the coxsackievirus and adenovirus receptor (CXADR) for virus entry to the cells. We prepared CXADR-knockout canine kidney cells and found that replication of BtAdVs was significantly hampered in these cells. For confirmation, their replication in canine CXADR-addback cells was rescued to the levels with the original cells. We also found that viral replication was corrected in human or bat CXADR-transduced cells to similar levels as in canine CXADR-addback cells. These results suggest that BtAdVs were able to use several mammalian-derived CXADRs as entry factors.

UR - http://www.scopus.com/inward/record.url?scp=85060541698&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060541698&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-37224-z

DO - 10.1038/s41598-018-37224-z

M3 - Article

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 573

ER -