Characterization of a polyclonal cytolytic T lymphocyte response to human immunodeficiency virus in persons without clinical progression

N. M. Lubaki, S. C. Ray, B. Dhruva, T. C. Quinn, R. F. Siliciano, R. C. Bollinger

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A total of 82 human immunodeficiency virus (HIV)-l-specific cytolytic T lymphocyte (CTL) clones were isolated and characterized from 5 HIV-infected subjects, utilizing multiple HLA class I alleles. B62-restricted. HIV-1 gag- specific CTL clones isolated from a single blood sample from I subject used four different Vβ gene rearrangements. Multiple CTL clones could be isolated from the same time point directed against HIV-1 gag, nef, and env from 1 subject. A prospective analysis resulted in the isolation of CTL clones from 1 subject directed against multiple HIV-1 antigens, including the same highly conserved nef peptide, over a 1-year period, in the absence of detectable circulating viral plasma RNA. These data suggest that in some persons without clinical progression and low levels of circulating HIV-1, the CTL response is polyclonal, is directed against multiple HIV-1 proteins, including highly conserved peptides within these proteins, and is maintained over time.

Original languageEnglish (US)
Pages (from-to)1360-1367
Number of pages8
JournalJournal of Infectious Diseases
Volume175
Issue number6
StatePublished - 1997
Externally publishedYes

Fingerprint

HIV-1
HIV
T-Lymphocytes
Clone Cells
Human Immunodeficiency Virus Proteins
Peptides
Gene Rearrangement
Viral RNA
Alleles
Antigens
Proteins

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Lubaki, N. M., Ray, S. C., Dhruva, B., Quinn, T. C., Siliciano, R. F., & Bollinger, R. C. (1997). Characterization of a polyclonal cytolytic T lymphocyte response to human immunodeficiency virus in persons without clinical progression. Journal of Infectious Diseases, 175(6), 1360-1367.

Characterization of a polyclonal cytolytic T lymphocyte response to human immunodeficiency virus in persons without clinical progression. / Lubaki, N. M.; Ray, S. C.; Dhruva, B.; Quinn, T. C.; Siliciano, R. F.; Bollinger, R. C.

In: Journal of Infectious Diseases, Vol. 175, No. 6, 1997, p. 1360-1367.

Research output: Contribution to journalArticle

Lubaki, NM, Ray, SC, Dhruva, B, Quinn, TC, Siliciano, RF & Bollinger, RC 1997, 'Characterization of a polyclonal cytolytic T lymphocyte response to human immunodeficiency virus in persons without clinical progression', Journal of Infectious Diseases, vol. 175, no. 6, pp. 1360-1367.
Lubaki, N. M. ; Ray, S. C. ; Dhruva, B. ; Quinn, T. C. ; Siliciano, R. F. ; Bollinger, R. C. / Characterization of a polyclonal cytolytic T lymphocyte response to human immunodeficiency virus in persons without clinical progression. In: Journal of Infectious Diseases. 1997 ; Vol. 175, No. 6. pp. 1360-1367.
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