Characterization of a previously unidentified hemoglobin fraction

Satish K. Srivastava, Cecile Van Loon, Ernest Beutler

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The hemoglobin peaks designated as "unnamed", Hb1a+b, Hb A1c, Hb AGSSG, Hb A0, and Hb A2 were separated from human red cell hemolysate by CM-Sephadex chromatography. The first peak of Hb, unnamed, was found to have 1 mole of pyridoxal phosphate per mole of Hb tetramer. Treatment of hemolysate with pyridoxal phosphate increased the total amount of Hb in this peak without changing the ratio of pyridoxal phosphate to Hb. Substantial amounts of pyridoxal phosphate were not found in any other Hb fraction. Cord blood hemolysate, consisting mainly of Hb F is pyridoxylated to a much smaller extent than adult hemoglobin.

Original languageEnglish (US)
Pages (from-to)617-621
Number of pages5
JournalBBA - Protein Structure
Issue number3
StatePublished - Oct 31 1972
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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