Characterization of a previously unidentified hemoglobin fraction

Satish K. Srivastava; Cecile Van Loon; Ernest Beutler

doi:10.1016/0005-2795(72)90026-8

Characterization of a previously unidentified hemoglobin fraction

Satish K. Srivastava
, Cecile Van Loon
, Ernest Beutler

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The hemoglobin peaks designated as "unnamed", Hb_1a+b, Hb A_1c, Hb A_GSSG, Hb A₀, and Hb A₂ were separated from human red cell hemolysate by CM-Sephadex chromatography. The first peak of Hb, unnamed, was found to have 1 mole of pyridoxal phosphate per mole of Hb tetramer. Treatment of hemolysate with pyridoxal phosphate increased the total amount of Hb in this peak without changing the ratio of pyridoxal phosphate to Hb. Substantial amounts of pyridoxal phosphate were not found in any other Hb fraction. Cord blood hemolysate, consisting mainly of Hb F is pyridoxylated to a much smaller extent than adult hemoglobin.

Original language	English (US)
Pages (from-to)	617-621
Number of pages	5
Journal	BBA - Protein Structure
Volume	278
Issue number	3
DOIs	https://doi.org/10.1016/0005-2795(72)90026-8
State	Published - Oct 31 1972
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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10.1016/0005-2795(72)90026-8

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@article{1f64956e15124967a9c0d9b6c9065b4a,

title = "Characterization of a previously unidentified hemoglobin fraction",

abstract = "The hemoglobin peaks designated as {"}unnamed{"}, Hb1a+b, Hb A1c, Hb AGSSG, Hb A0, and Hb A2 were separated from human red cell hemolysate by CM-Sephadex chromatography. The first peak of Hb, unnamed, was found to have 1 mole of pyridoxal phosphate per mole of Hb tetramer. Treatment of hemolysate with pyridoxal phosphate increased the total amount of Hb in this peak without changing the ratio of pyridoxal phosphate to Hb. Substantial amounts of pyridoxal phosphate were not found in any other Hb fraction. Cord blood hemolysate, consisting mainly of Hb F is pyridoxylated to a much smaller extent than adult hemoglobin.",

author = "Srivastava, \{Satish K.\} and \{Van Loon\}, Cecile and Ernest Beutler",

note = "Funding Information: The other minor peaks found on CM-Sephadex chromatography of hemolysates do not contain pyridoxal-P and their structure remains to be elucidated. This work was supported in part by Grant HE07449 from the Department of Health, Education and Welfare, and in part by a supporting fund established in the name of the David Kraman Medical Research Fellowship•",

year = "1972",

month = oct,

day = "31",

doi = "10.1016/0005-2795(72)90026-8",

language = "English (US)",

volume = "278",

pages = "617--621",

journal = "BBA - Protein Structure",

issn = "0005-2795",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Characterization of a previously unidentified hemoglobin fraction

AU - Srivastava, Satish K.

AU - Van Loon, Cecile

AU - Beutler, Ernest

N1 - Funding Information: The other minor peaks found on CM-Sephadex chromatography of hemolysates do not contain pyridoxal-P and their structure remains to be elucidated. This work was supported in part by Grant HE07449 from the Department of Health, Education and Welfare, and in part by a supporting fund established in the name of the David Kraman Medical Research Fellowship•

PY - 1972/10/31

Y1 - 1972/10/31

N2 - The hemoglobin peaks designated as "unnamed", Hb1a+b, Hb A1c, Hb AGSSG, Hb A0, and Hb A2 were separated from human red cell hemolysate by CM-Sephadex chromatography. The first peak of Hb, unnamed, was found to have 1 mole of pyridoxal phosphate per mole of Hb tetramer. Treatment of hemolysate with pyridoxal phosphate increased the total amount of Hb in this peak without changing the ratio of pyridoxal phosphate to Hb. Substantial amounts of pyridoxal phosphate were not found in any other Hb fraction. Cord blood hemolysate, consisting mainly of Hb F is pyridoxylated to a much smaller extent than adult hemoglobin.

AB - The hemoglobin peaks designated as "unnamed", Hb1a+b, Hb A1c, Hb AGSSG, Hb A0, and Hb A2 were separated from human red cell hemolysate by CM-Sephadex chromatography. The first peak of Hb, unnamed, was found to have 1 mole of pyridoxal phosphate per mole of Hb tetramer. Treatment of hemolysate with pyridoxal phosphate increased the total amount of Hb in this peak without changing the ratio of pyridoxal phosphate to Hb. Substantial amounts of pyridoxal phosphate were not found in any other Hb fraction. Cord blood hemolysate, consisting mainly of Hb F is pyridoxylated to a much smaller extent than adult hemoglobin.

UR - https://www.scopus.com/pages/publications/0015531102

UR - https://www.scopus.com/pages/publications/0015531102#tab=citedBy

U2 - 10.1016/0005-2795(72)90026-8

DO - 10.1016/0005-2795(72)90026-8

M3 - Article

C2 - 5085674

AN - SCOPUS:0015531102

SN - 0005-2795

VL - 278

SP - 617

EP - 621

JO - BBA - Protein Structure

JF - BBA - Protein Structure

IS - 3

ER -

Characterization of a previously unidentified hemoglobin fraction

Abstract

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