Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy

Farzan Siddiqui, Chuan Yuan Li, Xiuwu Zhang, Susan M. Larue, Mark W. Dewhirst, Robert L. Ullrich, Paul R. Avery

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Interleukin-12 (IL-12) is a pro-inflammatory cytokine that has shown great promise as a therapeutic agent in experimental models of infectious disease and cancer. However, it is also a highly toxic molecule and for that reason has not been accepted readily into the clinic. A replication-deficient adenoviral vector was designed to deliver the feline interleukin-12 gene into tumour cells. The interleukin-12 gene has been placed under control of a heat inducible promoter, human heat shock promoter 70b, with the intent of spatially and temporally controlling the expression of IL-12, thus limiting its toxicity. In vitro, the transfection efficiency of the adenoviral vector, the effect of multiplicity of infection and the production of biologically active feline IL-12 were studied in the infected cells in response to a range of temperatures. This adenoviral vector will be a useful tool to examine the effects of intra-tumoural IL-12 delivery in a spontaneously occurring feline soft tissue sarcoma model.

Original languageEnglish (US)
Pages (from-to)117-134
Number of pages18
JournalInternational Journal of Hyperthermia
Volume22
Issue number2
DOIs
StatePublished - Mar 1 2006

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Keywords

  • Adenovirus
  • Heat shock promoter
  • Hyperthermia
  • Interleukin-12

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cancer Research

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