Characterization of Adipose-Derived Stem Cells Following Burn Injury

Anesh Prasai, Amina El Ayadi, Randy C. Mifflin, Michael D. Wetzel, Clark R. Andersen, Heinz Redl, David Herndon, Celeste Finnerty

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Severe burns induce a prolonged inflammatory response in subcutaneous adipose tissue that modulates signaling in adipose-derived stem cells (ASCs), which hold potential for healing burn wounds or generating skin substitutes. Using a 60% rat scald burn model, we conducted a series of experiments to determine which cells isolated from the adipose tissue produced inflammatory mediators and how these changes affect ASC fate and function. The stromal vascular fraction (SVF), adipocytes, and ASCs were isolated from adipose tissue at varying times up to 4 weeks postburn and from non-injured controls. Endpoints included inflammatory marker expression, expression of ASC-specific cell-surface markers, DNA damage, differentiation potential, and proliferation. Inflammatory marker expression was induced in adipocytes and the SVF at 24 and 48 h postburn; expression of inflammatory marker mRNA transcripts and protein returned to normal in the SVF isolated 1 week postburn. In enriched ASCs, burns did not alter cell-surface expression of stem cell markers, markers of inflammation, differentiation potential, or proliferative ability. These results suggest that adipocytes and the SVF produce large quantities of inflammatory mediators, but that ASCs do not, after burns and that ASCs are unaffected by burn injury or culturing procedures.. They also suggest that cells isolated over 48 h after injury are best for cell culture or tissue engineering purposes.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalStem Cell Reviews and Reports
DOIs
StateAccepted/In press - Jun 23 2017

Fingerprint

Stem Cells
Wounds and Injuries
Blood Vessels
Burns
Adipocytes
Adipose Tissue
Artificial Skin
Cell Engineering
Subcutaneous Fat
Differentiation Antigens
Tissue Engineering
Wound Healing
DNA Damage
Cell Culture Techniques
Inflammation
Messenger RNA
Proteins

Keywords

  • Adipocytes
  • Adipose tissue
  • Adipose-derived stem cell
  • Burns
  • Inflammation
  • Stromal vascular fraction

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

Cite this

Characterization of Adipose-Derived Stem Cells Following Burn Injury. / Prasai, Anesh; El Ayadi, Amina; Mifflin, Randy C.; Wetzel, Michael D.; Andersen, Clark R.; Redl, Heinz; Herndon, David; Finnerty, Celeste.

In: Stem Cell Reviews and Reports, 23.06.2017, p. 1-12.

Research output: Contribution to journalArticle

Prasai, Anesh ; El Ayadi, Amina ; Mifflin, Randy C. ; Wetzel, Michael D. ; Andersen, Clark R. ; Redl, Heinz ; Herndon, David ; Finnerty, Celeste. / Characterization of Adipose-Derived Stem Cells Following Burn Injury. In: Stem Cell Reviews and Reports. 2017 ; pp. 1-12.
@article{c17508c895984168b33db3299660a8ab,
title = "Characterization of Adipose-Derived Stem Cells Following Burn Injury",
abstract = "Severe burns induce a prolonged inflammatory response in subcutaneous adipose tissue that modulates signaling in adipose-derived stem cells (ASCs), which hold potential for healing burn wounds or generating skin substitutes. Using a 60{\%} rat scald burn model, we conducted a series of experiments to determine which cells isolated from the adipose tissue produced inflammatory mediators and how these changes affect ASC fate and function. The stromal vascular fraction (SVF), adipocytes, and ASCs were isolated from adipose tissue at varying times up to 4 weeks postburn and from non-injured controls. Endpoints included inflammatory marker expression, expression of ASC-specific cell-surface markers, DNA damage, differentiation potential, and proliferation. Inflammatory marker expression was induced in adipocytes and the SVF at 24 and 48 h postburn; expression of inflammatory marker mRNA transcripts and protein returned to normal in the SVF isolated 1 week postburn. In enriched ASCs, burns did not alter cell-surface expression of stem cell markers, markers of inflammation, differentiation potential, or proliferative ability. These results suggest that adipocytes and the SVF produce large quantities of inflammatory mediators, but that ASCs do not, after burns and that ASCs are unaffected by burn injury or culturing procedures.. They also suggest that cells isolated over 48 h after injury are best for cell culture or tissue engineering purposes.",
keywords = "Adipocytes, Adipose tissue, Adipose-derived stem cell, Burns, Inflammation, Stromal vascular fraction",
author = "Anesh Prasai and {El Ayadi}, Amina and Mifflin, {Randy C.} and Wetzel, {Michael D.} and Andersen, {Clark R.} and Heinz Redl and David Herndon and Celeste Finnerty",
year = "2017",
month = "6",
day = "23",
doi = "10.1007/s12015-017-9721-9",
language = "English (US)",
pages = "1--12",
journal = "Stem Cell Reviews",
issn = "1550-8943",
publisher = "Humana Press",

}

TY - JOUR

T1 - Characterization of Adipose-Derived Stem Cells Following Burn Injury

AU - Prasai, Anesh

AU - El Ayadi, Amina

AU - Mifflin, Randy C.

AU - Wetzel, Michael D.

AU - Andersen, Clark R.

AU - Redl, Heinz

AU - Herndon, David

AU - Finnerty, Celeste

PY - 2017/6/23

Y1 - 2017/6/23

N2 - Severe burns induce a prolonged inflammatory response in subcutaneous adipose tissue that modulates signaling in adipose-derived stem cells (ASCs), which hold potential for healing burn wounds or generating skin substitutes. Using a 60% rat scald burn model, we conducted a series of experiments to determine which cells isolated from the adipose tissue produced inflammatory mediators and how these changes affect ASC fate and function. The stromal vascular fraction (SVF), adipocytes, and ASCs were isolated from adipose tissue at varying times up to 4 weeks postburn and from non-injured controls. Endpoints included inflammatory marker expression, expression of ASC-specific cell-surface markers, DNA damage, differentiation potential, and proliferation. Inflammatory marker expression was induced in adipocytes and the SVF at 24 and 48 h postburn; expression of inflammatory marker mRNA transcripts and protein returned to normal in the SVF isolated 1 week postburn. In enriched ASCs, burns did not alter cell-surface expression of stem cell markers, markers of inflammation, differentiation potential, or proliferative ability. These results suggest that adipocytes and the SVF produce large quantities of inflammatory mediators, but that ASCs do not, after burns and that ASCs are unaffected by burn injury or culturing procedures.. They also suggest that cells isolated over 48 h after injury are best for cell culture or tissue engineering purposes.

AB - Severe burns induce a prolonged inflammatory response in subcutaneous adipose tissue that modulates signaling in adipose-derived stem cells (ASCs), which hold potential for healing burn wounds or generating skin substitutes. Using a 60% rat scald burn model, we conducted a series of experiments to determine which cells isolated from the adipose tissue produced inflammatory mediators and how these changes affect ASC fate and function. The stromal vascular fraction (SVF), adipocytes, and ASCs were isolated from adipose tissue at varying times up to 4 weeks postburn and from non-injured controls. Endpoints included inflammatory marker expression, expression of ASC-specific cell-surface markers, DNA damage, differentiation potential, and proliferation. Inflammatory marker expression was induced in adipocytes and the SVF at 24 and 48 h postburn; expression of inflammatory marker mRNA transcripts and protein returned to normal in the SVF isolated 1 week postburn. In enriched ASCs, burns did not alter cell-surface expression of stem cell markers, markers of inflammation, differentiation potential, or proliferative ability. These results suggest that adipocytes and the SVF produce large quantities of inflammatory mediators, but that ASCs do not, after burns and that ASCs are unaffected by burn injury or culturing procedures.. They also suggest that cells isolated over 48 h after injury are best for cell culture or tissue engineering purposes.

KW - Adipocytes

KW - Adipose tissue

KW - Adipose-derived stem cell

KW - Burns

KW - Inflammation

KW - Stromal vascular fraction

UR - http://www.scopus.com/inward/record.url?scp=85021209257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021209257&partnerID=8YFLogxK

U2 - 10.1007/s12015-017-9721-9

DO - 10.1007/s12015-017-9721-9

M3 - Article

SP - 1

EP - 12

JO - Stem Cell Reviews

JF - Stem Cell Reviews

SN - 1550-8943

ER -