Characterization of Aeromonas hydrophila wound pathotypes by comparative genomic and functional analyses of virulence genes

Christopher J. Grim, Elena V. Kozlov, Jian Sha, Eric C. Fitts, Christina J. van Lier, Michelle L. Kirtley, Sandeep J. Joseph, Timothy D. Read, Eileen M. Burd, Ben D. Tall, Sam W. Joseph, Amy J. Horneman, Ashok Chopra, Joshua R. Shak

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Abstract

Aeromonas hydrophila has increasingly been implicated as a virulent and antibiotic-resistant etiologic agent in various human diseases. In a previously published case report, we described a subject with a polymicrobial wound infection that included a persistent and aggressive strain of A. hydrophila (E1), as well as a more antibiotic-resistant strain of A. hydrophila (E2). To better understand the differences between pathogenic and environmental strains of A. hydrophila, we conducted comparative genomic and functional analyses of virulence-associated genes of these two wound isolates (E1 and E2), the environmental type strain A. hydrophila ATCC 7966T, and four other isolates belonging to A. aquariorum, A. veronii, A. salmonicida, and A. caviae. Full-genome sequencing of strains E1 and E2 revealed extensive differences between the two and strain ATCC 7966T. The more persistent wound infection strain, E1, harbored coding sequences for a cytotoxic enterotoxin (Act), a type 3 secretion system (T3SS), flagella, hemolysins, and a homolog of exotoxin A found in Pseudomonas aeruginosa. Corresponding phenotypic analyses with A. hydrophila ATCC 7966T and SSU as reference strains demonstrated the functionality of these virulence genes, with strain E1 displaying enhanced swimming and swarming motility, lateral flagella on electron microscopy, the presence of T3SS effector AexU, and enhanced lethality in a mouse model of Aeromonas infection. By combining sequence-based analysis and functional assays, we characterized an A. hydrophila pathotype, exemplified by strain E1, that exhibited increased virulence in a mouse model of infection, likely because of encapsulation, enhanced motility, toxin secretion, and cellular toxicity.

Original languageEnglish (US)
Article numbere00064-13
JournalmBio
Volume4
Issue number2
DOIs
StatePublished - Mar 2013

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Aeromonas hydrophila
Virulence
Wounds and Injuries
Genes
Flagella
Wound Infection
Anti-Bacterial Agents
Aeromonas
Exotoxins
Hemolysin Proteins
Enterotoxins
Infection
Coinfection
Pseudomonas aeruginosa
Sequence Analysis
Electron Microscopy
Genome

ASJC Scopus subject areas

  • Microbiology
  • Virology

Cite this

Grim, C. J., Kozlov, E. V., Sha, J., Fitts, E. C., van Lier, C. J., Kirtley, M. L., ... Shak, J. R. (2013). Characterization of Aeromonas hydrophila wound pathotypes by comparative genomic and functional analyses of virulence genes. mBio, 4(2), [e00064-13]. https://doi.org/10.1128/mBio.00064-13

Characterization of Aeromonas hydrophila wound pathotypes by comparative genomic and functional analyses of virulence genes. / Grim, Christopher J.; Kozlov, Elena V.; Sha, Jian; Fitts, Eric C.; van Lier, Christina J.; Kirtley, Michelle L.; Joseph, Sandeep J.; Read, Timothy D.; Burd, Eileen M.; Tall, Ben D.; Joseph, Sam W.; Horneman, Amy J.; Chopra, Ashok; Shak, Joshua R.

In: mBio, Vol. 4, No. 2, e00064-13, 03.2013.

Research output: Contribution to journalArticle

Grim, CJ, Kozlov, EV, Sha, J, Fitts, EC, van Lier, CJ, Kirtley, ML, Joseph, SJ, Read, TD, Burd, EM, Tall, BD, Joseph, SW, Horneman, AJ, Chopra, A & Shak, JR 2013, 'Characterization of Aeromonas hydrophila wound pathotypes by comparative genomic and functional analyses of virulence genes', mBio, vol. 4, no. 2, e00064-13. https://doi.org/10.1128/mBio.00064-13
Grim, Christopher J. ; Kozlov, Elena V. ; Sha, Jian ; Fitts, Eric C. ; van Lier, Christina J. ; Kirtley, Michelle L. ; Joseph, Sandeep J. ; Read, Timothy D. ; Burd, Eileen M. ; Tall, Ben D. ; Joseph, Sam W. ; Horneman, Amy J. ; Chopra, Ashok ; Shak, Joshua R. / Characterization of Aeromonas hydrophila wound pathotypes by comparative genomic and functional analyses of virulence genes. In: mBio. 2013 ; Vol. 4, No. 2.
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abstract = "Aeromonas hydrophila has increasingly been implicated as a virulent and antibiotic-resistant etiologic agent in various human diseases. In a previously published case report, we described a subject with a polymicrobial wound infection that included a persistent and aggressive strain of A. hydrophila (E1), as well as a more antibiotic-resistant strain of A. hydrophila (E2). To better understand the differences between pathogenic and environmental strains of A. hydrophila, we conducted comparative genomic and functional analyses of virulence-associated genes of these two wound isolates (E1 and E2), the environmental type strain A. hydrophila ATCC 7966T, and four other isolates belonging to A. aquariorum, A. veronii, A. salmonicida, and A. caviae. Full-genome sequencing of strains E1 and E2 revealed extensive differences between the two and strain ATCC 7966T. The more persistent wound infection strain, E1, harbored coding sequences for a cytotoxic enterotoxin (Act), a type 3 secretion system (T3SS), flagella, hemolysins, and a homolog of exotoxin A found in Pseudomonas aeruginosa. Corresponding phenotypic analyses with A. hydrophila ATCC 7966T and SSU as reference strains demonstrated the functionality of these virulence genes, with strain E1 displaying enhanced swimming and swarming motility, lateral flagella on electron microscopy, the presence of T3SS effector AexU, and enhanced lethality in a mouse model of Aeromonas infection. By combining sequence-based analysis and functional assays, we characterized an A. hydrophila pathotype, exemplified by strain E1, that exhibited increased virulence in a mouse model of infection, likely because of encapsulation, enhanced motility, toxin secretion, and cellular toxicity.",
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