Characterization of an actin-targeting ADP-ribosyltransferase from Aeromonas hydrophila

Adin Shniffer, Danielle D. Visschedyk, Ravikiran Ravulapalli, Giovanni Suarez, Zachari J. Turgeon, Anthony A. Petrie, Ashok Chopra, A. Rod Merrill

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: VahC toxin from Aeromonas hydrophila inactivates actin by transferring ADP-ribose from NAD+. Results: The crystal structure of VahC reveals new insights into the interaction of the actin substrate with the actin-targeting toxins, and consequently, potent inhibitors have been developed. Conclusion: Broad spectrum inhibitors have been developed against actin-targeting toxins from the ADP-ribosyltransferase family. Significance: This new insight provides impetus for therapeutic development for treating bacterial diseases.

Original languageEnglish (US)
Pages (from-to)37030-37041
Number of pages12
JournalJournal of Biological Chemistry
Volume287
Issue number44
DOIs
StatePublished - Oct 26 2012

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ADP Ribose Transferases
Aeromonas hydrophila
Actins
Adenosine Diphosphate Ribose
NAD
Crystal structure
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Shniffer, A., Visschedyk, D. D., Ravulapalli, R., Suarez, G., Turgeon, Z. J., Petrie, A. A., ... Merrill, A. R. (2012). Characterization of an actin-targeting ADP-ribosyltransferase from Aeromonas hydrophila. Journal of Biological Chemistry, 287(44), 37030-37041. https://doi.org/10.1074/jbc.M112.397612

Characterization of an actin-targeting ADP-ribosyltransferase from Aeromonas hydrophila. / Shniffer, Adin; Visschedyk, Danielle D.; Ravulapalli, Ravikiran; Suarez, Giovanni; Turgeon, Zachari J.; Petrie, Anthony A.; Chopra, Ashok; Merrill, A. Rod.

In: Journal of Biological Chemistry, Vol. 287, No. 44, 26.10.2012, p. 37030-37041.

Research output: Contribution to journalArticle

Shniffer, A, Visschedyk, DD, Ravulapalli, R, Suarez, G, Turgeon, ZJ, Petrie, AA, Chopra, A & Merrill, AR 2012, 'Characterization of an actin-targeting ADP-ribosyltransferase from Aeromonas hydrophila', Journal of Biological Chemistry, vol. 287, no. 44, pp. 37030-37041. https://doi.org/10.1074/jbc.M112.397612
Shniffer A, Visschedyk DD, Ravulapalli R, Suarez G, Turgeon ZJ, Petrie AA et al. Characterization of an actin-targeting ADP-ribosyltransferase from Aeromonas hydrophila. Journal of Biological Chemistry. 2012 Oct 26;287(44):37030-37041. https://doi.org/10.1074/jbc.M112.397612
Shniffer, Adin ; Visschedyk, Danielle D. ; Ravulapalli, Ravikiran ; Suarez, Giovanni ; Turgeon, Zachari J. ; Petrie, Anthony A. ; Chopra, Ashok ; Merrill, A. Rod. / Characterization of an actin-targeting ADP-ribosyltransferase from Aeromonas hydrophila. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 44. pp. 37030-37041.
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