Characterization of an antiviral agent from primary murine fibroblast cultures

murine tissue culture CVI.

M. L. Sullivan, David Niesel, D. H. Coppenhaver, J. Sabados, S. Baron

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have previously described a class of virus inhibitors which are produced spontaneously by many types of cells in culture and present in a number of physiological fluids. These inhibitors are differentiated from all other known naturally occurring antiviral substances in regard to their (i) lack of species specificity, (ii) broad antiviral activity (iii) absence of high affinity binding by the inhibitor to the virus, (iv) mechanism of the action of the inhibitor is through inhibition of viral attachment, and (v) extreme thermal stability. In this report, we show that this class of inhibitors can be divided into two distinct subclasses. The first category includes the inhibitor spontaneously produced by cells in culture, originally described as contact-blocking viral inhibitor (CVI), and has a polypeptide component associated with its antiviral activity. The second category includes the inhibitors detected in body fluids and tissue extracts and has no essential peptide structure. Further characterization of CVI with respect to molecular size and stability to heat and a number of chemical reagents and enzymes indicate that the antiviral activity of CVI is associated with a large molecule (90s or approximately 4 million daltons), is stable at 100(8)C, and is resistant to the action of RNase, DNase, sulfhydral reagents, protein denaturants, and extraction by organic solvents.

Original languageEnglish (US)
Pages (from-to)126-132
Number of pages7
JournalJournal of Biological Regulators and Homeostatic Agents
Volume1
Issue number3
StatePublished - Jul 1987

Fingerprint

antiviral agents
Antiviral Agents
fibroblasts
tissue culture
cell culture
Fibroblasts
viruses
deoxyribonucleases
body fluids
mice
thermal stability
mechanism of action
polypeptides
Extreme Heat
Cell Culture Techniques
peptides
Viruses
heat
Species Specificity
Peptides

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Endocrinology
  • Physiology
  • Immunology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)

Cite this

Characterization of an antiviral agent from primary murine fibroblast cultures : murine tissue culture CVI. / Sullivan, M. L.; Niesel, David; Coppenhaver, D. H.; Sabados, J.; Baron, S.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 1, No. 3, 07.1987, p. 126-132.

Research output: Contribution to journalArticle

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