Characterization of bovine homologues of granulysin and NK-lysin

Janice Endsley, Jason L. Furrer, Mark Endsley, Mark A. McIntosh, Alexander C. Maue, W. Ray Waters, David R. Lee, D. Mark Estes

Research output: Contribution to journalArticle

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Abstract

Granulysin and NK-lysin are antimicrobial proteins found in the granules of human and swine cytotoxic lymphocytes. A murine counterpart to granulysin has not been identified to date, indicating the importance of additional models to fully characterize the role of granulysin-like molecules in the immune response to infectious disease. Two partial nucleotide sequences corresponding to the complete functional domain of granulysin and NK-lysin were amplified from bovine PBMC mRNA. Following stimulation with phorbol ester and calcium ionophore, expression of the bovine gene was detected in CD3+ T cells, CD4 + T cells, CD8+ T cells, WC1+ γδ T cells, and PBMC depleted of CD3+ T cells, but was absent in CD21 + cells and CD14+ cells. Intracellular flow cytometry and immunoblotting confirmed the presence of protein corresponding to the bovine granulysin homologue in activated T lymphocytes and PBMC. Synthetic human, bovine, and swine peptides corresponding to the C terminus of helix 2 through helix 3 region of granulysin displayed potent antimicrobial activity against Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, and Mycobacterium bovis bacillus Calmette-Guérin. Human and bovine peptides corresponding to helix 2 displayed antimycobacterial activity against M. bovis bacillus Calmette-Guérin. Expression of the bovine gene was detected in laser microscopy-dissected lymph node lesions from an M. bovis-infected animal. The identification of a biologically active bovine homologue to granulysin demonstrates the potential of the bovine model in characterizing the role of granulysin in the immune response to a variety of infectious agents.

Original languageEnglish (US)
Pages (from-to)2607-2614
Number of pages8
JournalJournal of Immunology
Volume173
Issue number4
StatePublished - Aug 15 2004

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T-Lymphocytes
Mycobacterium bovis
Bacillus
Swine
Gene Expression
Salmonella enteritidis
Peptides
Calcium Ionophores
NK-lysin
Phorbol Esters
Immunoblotting
Confocal Microscopy
Communicable Diseases
Staphylococcus aureus
Flow Cytometry
Proteins
Lymph Nodes
Lymphocytes
Escherichia coli
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Endsley, J., Furrer, J. L., Endsley, M., McIntosh, M. A., Maue, A. C., Waters, W. R., ... Estes, D. M. (2004). Characterization of bovine homologues of granulysin and NK-lysin. Journal of Immunology, 173(4), 2607-2614.

Characterization of bovine homologues of granulysin and NK-lysin. / Endsley, Janice; Furrer, Jason L.; Endsley, Mark; McIntosh, Mark A.; Maue, Alexander C.; Waters, W. Ray; Lee, David R.; Estes, D. Mark.

In: Journal of Immunology, Vol. 173, No. 4, 15.08.2004, p. 2607-2614.

Research output: Contribution to journalArticle

Endsley, J, Furrer, JL, Endsley, M, McIntosh, MA, Maue, AC, Waters, WR, Lee, DR & Estes, DM 2004, 'Characterization of bovine homologues of granulysin and NK-lysin', Journal of Immunology, vol. 173, no. 4, pp. 2607-2614.
Endsley J, Furrer JL, Endsley M, McIntosh MA, Maue AC, Waters WR et al. Characterization of bovine homologues of granulysin and NK-lysin. Journal of Immunology. 2004 Aug 15;173(4):2607-2614.
Endsley, Janice ; Furrer, Jason L. ; Endsley, Mark ; McIntosh, Mark A. ; Maue, Alexander C. ; Waters, W. Ray ; Lee, David R. ; Estes, D. Mark. / Characterization of bovine homologues of granulysin and NK-lysin. In: Journal of Immunology. 2004 ; Vol. 173, No. 4. pp. 2607-2614.
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