Characterization of bovine pulmonary and serum antibody responses after parenteral or intrapulmonary vaccination with live Pasteurella haemolytica

Jere McBride, Richard E. Corstvet, Daniel B. Paulsen, J. Raymond McClure, Fred M. Enright

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pulmonary and serum antibody responses were evaluated in eight calves vaccinated [four intrapulmonary-right diaphragmatic lobe (IP) and four subcutaneous (SC)] with Pasteurella haemolytica Al (Ph-1) impregnated agar beads and eight respective sham-vaccinated calves. Experimental and sham groups were challenged in both diaphragmatic lobes with Ph-1 34-37 d after vaccination (DAV) and necropsied 6 d after challenge (DAC; 40-43 DAV). IgG antibodies contained in fluids from the diaphragmatic lobes of vaccinated calves had different patterns of antigen specificity compared with IgG antibodies in analogous sera. Using ELISA, anti-Ph-1 IgA and IgG antibody concentrations were significantly higher (P < 0.05) in lung lavage fluids from the IP group before and after challenge compared to the SC and sham groups. The IP and SC groups developed IgA, IgG and IgM antibody titers in nonvaccinated lung lobes after vaccination and challenge. The IP and SC groups exhibited significantly (P < 0.05) smaller pulmonary lesions than the sham groups and pulmonary IgG and IgA antibodies were associated with increased protection.

Original languageEnglish (US)
Pages (from-to)99-115
Number of pages17
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume19
Issue number2
DOIs
StatePublished - Feb 1996
Externally publishedYes

Fingerprint

Mannheimia haemolytica
Antibody Formation
Vaccination
Immunoglobulin G
lungs
vaccination
Lung
antibodies
Antibodies
cattle
Immunoglobulin A
Serum
calves
Bronchoalveolar Lavage Fluid
Agar
Immunoglobulin M
lesions (animal)
Enzyme-Linked Immunosorbent Assay
Antigens
agar

Keywords

  • Bovine
  • Lymphocytes
  • Pasteurella haemolytica
  • Pulmonary antibody
  • Serum antibody

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • Microbiology
  • veterinary(all)

Cite this

Characterization of bovine pulmonary and serum antibody responses after parenteral or intrapulmonary vaccination with live Pasteurella haemolytica. / McBride, Jere; Corstvet, Richard E.; Paulsen, Daniel B.; McClure, J. Raymond; Enright, Fred M.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 19, No. 2, 02.1996, p. 99-115.

Research output: Contribution to journalArticle

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