Characterization of coronavirus DI RNA packaging

K. H. Kim, K. Narayanan, Shinji Makino

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Studies of defective interfering (DI) RNAs of mouse hepatitis virus (MHV), suggest that a 69 nt-long packaging signal, which is located about 20 kb from the 5'-end of the 31 kb-long MHV genomic RNA, is necessary and sufficient for MHV genomic RNA packaging into MHV particles. We demonstrated that use of a low pH culture medium combined with subsequent ultrafiltration increased MHV infectivity about 60 times over MHV preparations grown in neutral medium. Using this virus concentration procedure, we successfully prepared DI particle-rich MHV preparations. Characterization of virus samples released from the cells infected with DI particle-rich MHV revealed that infectious MHV genomic RNA was not required for packaging of DI RNAs. These data suggested that interaction of the DI packaging signal with an unidentified region(s) of helper virus genomic RNA is unlikely, and therefore unlikely to facilitate the packaging of MHV DI RNA into the MHV virion. Rather, both DI RNA and MHV genomic RNA probably use the packaging signal for RNA packaging.

Original languageEnglish (US)
Pages (from-to)347-353
Number of pages7
JournalAdvances in Experimental Medicine and Biology
Volume440
StatePublished - 1998
Externally publishedYes

Fingerprint

Murine hepatitis virus
Coronavirus
Product Packaging
Viruses
Packaging
RNA
Defective Viruses
Virion
Hepatovirus
Helper Viruses
Ultrafiltration
Culture Media

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Characterization of coronavirus DI RNA packaging. / Kim, K. H.; Narayanan, K.; Makino, Shinji.

In: Advances in Experimental Medicine and Biology, Vol. 440, 1998, p. 347-353.

Research output: Contribution to journalArticle

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