Characterization of DNA glycosylase activity by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Agus Darwanto, Alvin Farrel, Daniel K. Rogstad, Lawrence Sowers

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The DNA of all organisms is persistently damaged by endogenous reactive molecules. Most of the single-base endogenous damage is repaired through the base excision repair (BER) pathway that is initiated by members of the DNA glycosylase family. Although the BER pathway is often considered to proceed through a common abasic site intermediate, emerging evidence indicates that there are likely distinct branches reflected by the multitude of chemically different 3′ and 5′ ends generated at the repair site. In this study, we have applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to the analysis of model DNA substrates acted on by recombinant glycosylases. We examine the chemical identity of several possible abasic site and nicked intermediates generated by monofunctional and bifunctional glycosylases. Our results suggest that the intermediate from endoIII/Nth might not be a simple β-elimination product as described previously. On the basis of 18O incorporation experiments, we propose a new mechanism for the endoIII/Nth family of glycosylases that may resolve several of the previous controversies. We further demonstrate that the use of an array of lesion-containing oligonucleotides can be used to rapidly examine the substrate preferences of a given glycosylase. Some of the lesions examined here can be acted on by more than one glycosylase, resulting in a spectrum of damaged intermediates for each lesion, suggesting that the sequence and coordination of repair activities that act on these lesions may influence the biological outcome of damage repair.

Original languageEnglish (US)
Pages (from-to)13-23
Number of pages11
JournalAnalytical Biochemistry
Volume394
Issue number1
DOIs
StatePublished - Nov 1 2009
Externally publishedYes

Fingerprint

DNA Glycosylases
DNA Repair
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Repair
DNA
Oligonucleotides
Substrates
Molecules
Experiments

Keywords

  • DNA glycosylase
  • MALDI-TOF-MS
  • Oxidative damage
  • Substrate specificity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of DNA glycosylase activity by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. / Darwanto, Agus; Farrel, Alvin; Rogstad, Daniel K.; Sowers, Lawrence.

In: Analytical Biochemistry, Vol. 394, No. 1, 01.11.2009, p. 13-23.

Research output: Contribution to journalArticle

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