Characterization of Dobrava virus: A hantavirus from Slovenia, Yugoslavia

Tatjana Avsic‐Zupanc, Shu‐Yuan ‐Y Xiao, Radivoj Stojanovic, Ana Gligic, G. van der Groen, James W. Leduc

Research output: Contribution to journalArticlepeer-review

195 Scopus citations


Small mammals were collected in natural foci of hemorrhagic fever with renal syndrome (HFRS) in Slovenia, Yugoslavia, and a hantavirus was isolated from the lungs of an Apodemus flavicol lis captured in Dobrava village. This new isolate, Dobrava virus, was compared with representative strains of the Hantavirus genus by serological and polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) methods. It was found by cross immunofluorescent and enzyme‐linked immunosorbent assays that antigenic properties of Dobrava virus were different from those of other hantaviruses. The RNA of this virus was successfully amplified with hantavirus genus reactive primer sets by reverse transcriptase polymerase chain reaction (RT‐PCR); however, PCR‐RFLP analysis of the amplified product was shown to be unique among those of the known hantaviruses, further indicating that Dobrava virus represents a new hantavirus serotype. Published 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)132-137
Number of pages6
JournalJournal of Medical Virology
Issue number2
StatePublished - Oct 1992
Externally publishedYes


  • Dobrava virus
  • hemorrhagic fever with renal syndrome (HFRS)
  • polymerase chain reaction (PCR)
  • restriction fragment length polymorphism (RFLP)
  • virus isolation

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases


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