Characterization of Dobrava virus

A Hantavirus from Slovenia, Yugoslavia

T. Avsic-Zupanc, S. Y. Xiao, R. Stojanovic, A. Gligic, G. Van der Groen, James LeDuc

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

Small mammals were collected in natural foci of hemorrhagic fever with renal syndrome (HFRS) in Slovenia, Yugoslavia, and a hantavirus was isolated from the lungs of an Apodemus flavicol lis captured in Dobrava village. This new isolate, Dobrava virus, was compared with representative strains of the Hantavirus genus by serological and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. It was found by cross immunofluorescent and enzyme-linked immunosorbent assays that antigenic properties of Dobrava virus were different from those of other hantaviruses. The RNA of this virus was successfully amplified with hantavirus genus reactive primer sets by reverse transcriptase polymerase chain reaction (RT-PCR); however, PCR-RFLP analysis of the amplified product was shown to be unique among those of the known hantaviruses, further indicating that Dobrava virus represents a new hantavirus serotype.

Original languageEnglish (US)
Pages (from-to)132-137
Number of pages6
JournalJournal of Medical Virology
Volume38
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Hantavirus
Slovenia
Yugoslavia
Viruses
Restriction Fragment Length Polymorphisms
Hemorrhagic Fever with Renal Syndrome
Murinae
Polymerase Chain Reaction
RNA Viruses
Reverse Transcriptase Polymerase Chain Reaction
Mammals
Enzyme-Linked Immunosorbent Assay
Lung

Keywords

  • Dobrava virus
  • Hemorrhagic fever with renal syndrome (HFRS)
  • Polymerase chain reaction (PCR)
  • Restriction fragment length polymorphism (RFLP)
  • Virus isolation

ASJC Scopus subject areas

  • Virology

Cite this

Avsic-Zupanc, T., Xiao, S. Y., Stojanovic, R., Gligic, A., Van der Groen, G., & LeDuc, J. (1992). Characterization of Dobrava virus: A Hantavirus from Slovenia, Yugoslavia. Journal of Medical Virology, 38(2), 132-137. https://doi.org/10.1002/jmv.1890380211

Characterization of Dobrava virus : A Hantavirus from Slovenia, Yugoslavia. / Avsic-Zupanc, T.; Xiao, S. Y.; Stojanovic, R.; Gligic, A.; Van der Groen, G.; LeDuc, James.

In: Journal of Medical Virology, Vol. 38, No. 2, 1992, p. 132-137.

Research output: Contribution to journalArticle

Avsic-Zupanc, T, Xiao, SY, Stojanovic, R, Gligic, A, Van der Groen, G & LeDuc, J 1992, 'Characterization of Dobrava virus: A Hantavirus from Slovenia, Yugoslavia', Journal of Medical Virology, vol. 38, no. 2, pp. 132-137. https://doi.org/10.1002/jmv.1890380211
Avsic-Zupanc, T. ; Xiao, S. Y. ; Stojanovic, R. ; Gligic, A. ; Van der Groen, G. ; LeDuc, James. / Characterization of Dobrava virus : A Hantavirus from Slovenia, Yugoslavia. In: Journal of Medical Virology. 1992 ; Vol. 38, No. 2. pp. 132-137.
@article{08e060409b2b40b48ed1a7aca741a160,
title = "Characterization of Dobrava virus: A Hantavirus from Slovenia, Yugoslavia",
abstract = "Small mammals were collected in natural foci of hemorrhagic fever with renal syndrome (HFRS) in Slovenia, Yugoslavia, and a hantavirus was isolated from the lungs of an Apodemus flavicol lis captured in Dobrava village. This new isolate, Dobrava virus, was compared with representative strains of the Hantavirus genus by serological and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. It was found by cross immunofluorescent and enzyme-linked immunosorbent assays that antigenic properties of Dobrava virus were different from those of other hantaviruses. The RNA of this virus was successfully amplified with hantavirus genus reactive primer sets by reverse transcriptase polymerase chain reaction (RT-PCR); however, PCR-RFLP analysis of the amplified product was shown to be unique among those of the known hantaviruses, further indicating that Dobrava virus represents a new hantavirus serotype.",
keywords = "Dobrava virus, Hemorrhagic fever with renal syndrome (HFRS), Polymerase chain reaction (PCR), Restriction fragment length polymorphism (RFLP), Virus isolation",
author = "T. Avsic-Zupanc and Xiao, {S. Y.} and R. Stojanovic and A. Gligic and {Van der Groen}, G. and James LeDuc",
year = "1992",
doi = "10.1002/jmv.1890380211",
language = "English (US)",
volume = "38",
pages = "132--137",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Characterization of Dobrava virus

T2 - A Hantavirus from Slovenia, Yugoslavia

AU - Avsic-Zupanc, T.

AU - Xiao, S. Y.

AU - Stojanovic, R.

AU - Gligic, A.

AU - Van der Groen, G.

AU - LeDuc, James

PY - 1992

Y1 - 1992

N2 - Small mammals were collected in natural foci of hemorrhagic fever with renal syndrome (HFRS) in Slovenia, Yugoslavia, and a hantavirus was isolated from the lungs of an Apodemus flavicol lis captured in Dobrava village. This new isolate, Dobrava virus, was compared with representative strains of the Hantavirus genus by serological and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. It was found by cross immunofluorescent and enzyme-linked immunosorbent assays that antigenic properties of Dobrava virus were different from those of other hantaviruses. The RNA of this virus was successfully amplified with hantavirus genus reactive primer sets by reverse transcriptase polymerase chain reaction (RT-PCR); however, PCR-RFLP analysis of the amplified product was shown to be unique among those of the known hantaviruses, further indicating that Dobrava virus represents a new hantavirus serotype.

AB - Small mammals were collected in natural foci of hemorrhagic fever with renal syndrome (HFRS) in Slovenia, Yugoslavia, and a hantavirus was isolated from the lungs of an Apodemus flavicol lis captured in Dobrava village. This new isolate, Dobrava virus, was compared with representative strains of the Hantavirus genus by serological and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. It was found by cross immunofluorescent and enzyme-linked immunosorbent assays that antigenic properties of Dobrava virus were different from those of other hantaviruses. The RNA of this virus was successfully amplified with hantavirus genus reactive primer sets by reverse transcriptase polymerase chain reaction (RT-PCR); however, PCR-RFLP analysis of the amplified product was shown to be unique among those of the known hantaviruses, further indicating that Dobrava virus represents a new hantavirus serotype.

KW - Dobrava virus

KW - Hemorrhagic fever with renal syndrome (HFRS)

KW - Polymerase chain reaction (PCR)

KW - Restriction fragment length polymorphism (RFLP)

KW - Virus isolation

UR - http://www.scopus.com/inward/record.url?scp=0026728094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026728094&partnerID=8YFLogxK

U2 - 10.1002/jmv.1890380211

DO - 10.1002/jmv.1890380211

M3 - Article

VL - 38

SP - 132

EP - 137

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 2

ER -