Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system

Alaina A. Brinley, Corey A. Theriot, Mayra Nelman-Gonzalez, Brian Crucian, Raymond P. Stowe, Alan Barrett, Duane L. Pierson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is the causative agent of mononucleosis and is also associated with several malignancies, including Burkitt's lymphoma, Hodgkin's lymphoma, and nasopharyngeal carcinoma, among others. EBV reactivates during spaceflight, with EBV shedding in saliva increasing to levels ten times those observed pre-and post-flight. Although stress has been shown to increase reactivation of EBV, other factors such as radiation and microgravity have been hypothesized to contribute to reactivation in space. We used a modeled spaceflight environment to evaluate the influence of radiation and microgravity on EBV reactivation. BJAB (EBV-negative) and Raji (EBV-positive) cell lines were assessed for viability/apoptosis, viral antigen and reactive oxygen species expression, and DNA damage and repair. EBV-infected cells did not experience decreased viability and increased apoptosis due to modeled spaceflight, whereas an EBV-negative cell line did, suggesting that EBV infection provided protection against apoptosis and cell death. Radiation was the major contributor to EBV ZEBRA upregulation. Combining modeled microgravity and radiation increased DNA damage and reactive oxygen species while modeled microgravity alone decreased DNA repair in Raji cells. Additionally, EBV-infected cells had increased DNA damage compared to EBV-negative cells. Since EBV-infected cells do not undergo apoptosis as readily as uninfected cells, it is possible that virus-infected cells in EBV seropositive individuals may have an increased risk to accumulate DNA damage during spaceflight. More studies are warranted to investigate this possibility. J. Cell. Biochem. 114: 616-624, 2013.

Original languageEnglish (US)
Pages (from-to)616-624
Number of pages9
JournalJournal of Cellular Biochemistry
Volume114
Issue number3
DOIs
StatePublished - Mar 2013

Fingerprint

Space Flight
Human Herpesvirus 4
Viruses
Weightlessness
Microgravity
DNA Damage
Radiation
Apoptosis
DNA
DNA Repair
Reactive Oxygen Species
Repair
Virus Shedding
Cells
Cell Line
Epstein-Barr Virus Infections
Burkitt Lymphoma
Viral Antigens
Hodgkin Disease
Saliva

Keywords

  • BIOREACTOR
  • EBV
  • EPSTEIN-BARR VIRUS
  • MICROGRAVITY
  • RADIATION
  • REACTIVATION

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Brinley, A. A., Theriot, C. A., Nelman-Gonzalez, M., Crucian, B., Stowe, R. P., Barrett, A., & Pierson, D. L. (2013). Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system. Journal of Cellular Biochemistry, 114(3), 616-624. https://doi.org/10.1002/jcb.24403

Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system. / Brinley, Alaina A.; Theriot, Corey A.; Nelman-Gonzalez, Mayra; Crucian, Brian; Stowe, Raymond P.; Barrett, Alan; Pierson, Duane L.

In: Journal of Cellular Biochemistry, Vol. 114, No. 3, 03.2013, p. 616-624.

Research output: Contribution to journalArticle

Brinley, AA, Theriot, CA, Nelman-Gonzalez, M, Crucian, B, Stowe, RP, Barrett, A & Pierson, DL 2013, 'Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system', Journal of Cellular Biochemistry, vol. 114, no. 3, pp. 616-624. https://doi.org/10.1002/jcb.24403
Brinley, Alaina A. ; Theriot, Corey A. ; Nelman-Gonzalez, Mayra ; Crucian, Brian ; Stowe, Raymond P. ; Barrett, Alan ; Pierson, Duane L. / Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system. In: Journal of Cellular Biochemistry. 2013 ; Vol. 114, No. 3. pp. 616-624.
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