Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment

Don L. Tucker, C. Mark Ott, Stephen Huff, Yuriy Fofanov, Duane L. Pierson, Richard C. Willson, George E. Fox

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background. Extra-cellular shear force is an important environmental parameter that is significant both medically and in the space environment. Escherichia coli cells grown in a low-shear modeled microgravity (LSMMG) environment produced in a high aspect rotating vessel (HARV) were subjected to transcriptional and physiological analysis. Results. Aerobic LSMMG cultures were grown in rich (LB) and minimal (MOPS + glucose) medium with a normal gravity vector HARV control. Reproducible changes in transcription were seen, but no specific LSMMG responsive genes were identified. Instead, absence of shear and a randomized gravity vector appears to cause local extra-cellular environmental changes, which elicit reproducible cellular responses. In minimal media, the majority of the significantly up- or down-regulated genes of known function were associated with the cell envelope. In rich medium, most LSMMG down-regulated genes were involved in translation. No observable changes in post-culture stress responses and antibiotic sensitivity were seen in cells immediately after exposure to LSMMG. Comparison with earlier studies of Salmonella enterica serovar Typhimurium conducted under similar growth conditions, revealed essentially no similarity in the genes that were significantly up- or down-regulated. Conclusion. Comparison of these results to previous studies suggests that different organisms may dramatically differ in their responses to medically significant low-shear and space environments. Depending on their specific response, some organisms, such as Salmonella, may become preadapted in a manner that predisposes them to increased virulence.

Original languageEnglish (US)
Article number15
JournalBMC Microbiology
Volume7
DOIs
StatePublished - 2007
Externally publishedYes

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Weightlessness
Escherichia coli
Genes
Hypergravity
Salmonella enterica
Gravitation
Salmonella
Virulence
Anti-Bacterial Agents
Glucose
Growth

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment. / Tucker, Don L.; Ott, C. Mark; Huff, Stephen; Fofanov, Yuriy; Pierson, Duane L.; Willson, Richard C.; Fox, George E.

In: BMC Microbiology, Vol. 7, 15, 2007.

Research output: Contribution to journalArticle

Tucker, Don L. ; Ott, C. Mark ; Huff, Stephen ; Fofanov, Yuriy ; Pierson, Duane L. ; Willson, Richard C. ; Fox, George E. / Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment. In: BMC Microbiology. 2007 ; Vol. 7.
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