Characterization of excretory/secretory endopeptidase and metallo-aminopeptidases from Taenia crassiceps metacestodes

Salman Baig, Raymond T. Damian, Jorge Morales-Montor, Paula Olecki, Jamil Talhouk, Rayhan Hashmey, A. Clinton White

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cysticercosis is caused by Taenia spp. metacestodes, which must survive in the host tissues to complete their life cycle. Their survival depends on their control of host immune responses. Because many parasites use proteases to modulate host responses, we examined culture media from Taenia crassiceps metacestodes for protease activity using peptide substrates. We identified prominent aminopeptidase activity at neutral pH, which was inhibited by chelating agents and partially inhibited by the aminopeptidase inhibitor, bestatin. Endopeptidase substrates were optimally cleaved at slightly acidic pH and endopeptidase activity was inhibited by cysteine protease inhibitors. Gel filtration FPLC and subsequent visualization by silver staining revealed a metallo-aminopeptidase of molecular weight 21 kDa and cysteine proteases of Mr 70 and 64 kDA. Recombinant IL-2 was digested when incubated with parasite culture supernatants, but not with control media. IL-2 degradation was completely inhibited by 1,10 phenanthroline and partially inhibited by bestatin, suggesting that a metallo-aminopeptidase was responsible. Incubation of human IgG with culture supernatants resulted in complete degradation of IgG, which was blocked by cysteine protease inhibitors. These observations demonstrate that Taenia spp. metacestodes secrete a number of proteolytic enzymes, which may target molecules from the host immune system and assist in evasion of the host immune response.

Original languageEnglish (US)
Pages (from-to)983-987
Number of pages5
JournalJournal of Parasitology
Volume91
Issue number5
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Taenia crassiceps
metacestodes
Taenia
Endopeptidases
Aminopeptidases
aminopeptidases
proteinases
Cysteine Proteinase Inhibitors
Peptide Hydrolases
cysteine proteinase inhibitors
inhibitor
Interleukin-2
interleukin-2
Parasites
immune response
Immunoglobulin G
Immune Evasion
parasite
Cysticercosis
Silver Staining

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Characterization of excretory/secretory endopeptidase and metallo-aminopeptidases from Taenia crassiceps metacestodes. / Baig, Salman; Damian, Raymond T.; Morales-Montor, Jorge; Olecki, Paula; Talhouk, Jamil; Hashmey, Rayhan; White, A. Clinton.

In: Journal of Parasitology, Vol. 91, No. 5, 10.2005, p. 983-987.

Research output: Contribution to journalArticle

Baig, Salman ; Damian, Raymond T. ; Morales-Montor, Jorge ; Olecki, Paula ; Talhouk, Jamil ; Hashmey, Rayhan ; White, A. Clinton. / Characterization of excretory/secretory endopeptidase and metallo-aminopeptidases from Taenia crassiceps metacestodes. In: Journal of Parasitology. 2005 ; Vol. 91, No. 5. pp. 983-987.
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