Characterization of exposure to low levels of viable Penicillium chrysogenum conidia and allergic sensitization induced by a protease allergen extract from viable P. chrysogenum conidia in mice

Christopher J. Schwab, J. Danny Cooley, Trevor Brasel, Cynthia A. Jumper, Suzanne C. Graham, David C. Straus

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Previous evidence by our laboratory has shown that mice inoculated with viable Penicillium chrysogenum conidia or spores at levels comparable to those found in contaminated buildings induced spore antigen-specific allergic responses. We proposed that mice exposed to low levels of viable P. chrysogenum conidia would not develop allergic symptoms. We also hypothesized that the symptoms induced by high numbers of conidia were the result of sensitization to allergens released by the conidia. Methods: C57BL/6 and BALB/c mice were exposed to 1 × 102 viable P. chrysogenum conidia by intranasal instillation weekly for a period of 11 weeks. C57BL/6 mice were also sensitized to a viable P. chrysogenum conidia protease extract by intraperitoneal injections for a period of 6 weeks followed by intranasal challenge with protease extract, viable, or nonviable P. chrysogenum conidia for 2 weeks. Results: C57BL/6 mice inoculated with low numbers of conidia developed no significant lung inflammation or increased serum immunoglobulins. Mice sensitized to the protease extract and challenged with both protease extract and viable conidia produced significant increases in serum IgE and IgG1. Mice sensitized to and challenged with the protease extract developed significant eosinophilia and mucus hyperproduction as determined by bronchoalveolar lavage and histopathological examination of lung tissue. Conclusions: Mice did not develop allergic symptoms in response to challenge with low levels of P. chrysogenum conidia. Protease allergens from viable conidia induced specific allergic responses in mice, indicating the importance of P. chrysogenum conidia in allergic sensitization to the organism.

Original languageEnglish (US)
Pages (from-to)200-208
Number of pages9
JournalInternational Archives of Allergy and Immunology
Volume130
Issue number3
DOIs
StatePublished - 2003
Externally publishedYes

Fingerprint

Penicillium chrysogenum
Fungal Spores
Allergens
Peptide Hydrolases
Spores
Inbred C57BL Mouse
Eosinophilia
Bronchoalveolar Lavage
Mucus
Intraperitoneal Injections
Serum
Immunoglobulin E
Immunoglobulins

Keywords

  • Allergy
  • Conidia
  • Eosinophils
  • IgE
  • IgG1
  • Mold
  • Murine model
  • Penicillium chrysogenum
  • Sick building syndrome

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Characterization of exposure to low levels of viable Penicillium chrysogenum conidia and allergic sensitization induced by a protease allergen extract from viable P. chrysogenum conidia in mice. / Schwab, Christopher J.; Cooley, J. Danny; Brasel, Trevor; Jumper, Cynthia A.; Graham, Suzanne C.; Straus, David C.

In: International Archives of Allergy and Immunology, Vol. 130, No. 3, 2003, p. 200-208.

Research output: Contribution to journalArticle

Schwab, Christopher J. ; Cooley, J. Danny ; Brasel, Trevor ; Jumper, Cynthia A. ; Graham, Suzanne C. ; Straus, David C. / Characterization of exposure to low levels of viable Penicillium chrysogenum conidia and allergic sensitization induced by a protease allergen extract from viable P. chrysogenum conidia in mice. In: International Archives of Allergy and Immunology. 2003 ; Vol. 130, No. 3. pp. 200-208.
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abstract = "Background: Previous evidence by our laboratory has shown that mice inoculated with viable Penicillium chrysogenum conidia or spores at levels comparable to those found in contaminated buildings induced spore antigen-specific allergic responses. We proposed that mice exposed to low levels of viable P. chrysogenum conidia would not develop allergic symptoms. We also hypothesized that the symptoms induced by high numbers of conidia were the result of sensitization to allergens released by the conidia. Methods: C57BL/6 and BALB/c mice were exposed to 1 × 102 viable P. chrysogenum conidia by intranasal instillation weekly for a period of 11 weeks. C57BL/6 mice were also sensitized to a viable P. chrysogenum conidia protease extract by intraperitoneal injections for a period of 6 weeks followed by intranasal challenge with protease extract, viable, or nonviable P. chrysogenum conidia for 2 weeks. Results: C57BL/6 mice inoculated with low numbers of conidia developed no significant lung inflammation or increased serum immunoglobulins. Mice sensitized to the protease extract and challenged with both protease extract and viable conidia produced significant increases in serum IgE and IgG1. Mice sensitized to and challenged with the protease extract developed significant eosinophilia and mucus hyperproduction as determined by bronchoalveolar lavage and histopathological examination of lung tissue. Conclusions: Mice did not develop allergic symptoms in response to challenge with low levels of P. chrysogenum conidia. Protease allergens from viable conidia induced specific allergic responses in mice, indicating the importance of P. chrysogenum conidia in allergic sensitization to the organism.",
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T1 - Characterization of exposure to low levels of viable Penicillium chrysogenum conidia and allergic sensitization induced by a protease allergen extract from viable P. chrysogenum conidia in mice

AU - Schwab, Christopher J.

AU - Cooley, J. Danny

AU - Brasel, Trevor

AU - Jumper, Cynthia A.

AU - Graham, Suzanne C.

AU - Straus, David C.

PY - 2003

Y1 - 2003

N2 - Background: Previous evidence by our laboratory has shown that mice inoculated with viable Penicillium chrysogenum conidia or spores at levels comparable to those found in contaminated buildings induced spore antigen-specific allergic responses. We proposed that mice exposed to low levels of viable P. chrysogenum conidia would not develop allergic symptoms. We also hypothesized that the symptoms induced by high numbers of conidia were the result of sensitization to allergens released by the conidia. Methods: C57BL/6 and BALB/c mice were exposed to 1 × 102 viable P. chrysogenum conidia by intranasal instillation weekly for a period of 11 weeks. C57BL/6 mice were also sensitized to a viable P. chrysogenum conidia protease extract by intraperitoneal injections for a period of 6 weeks followed by intranasal challenge with protease extract, viable, or nonviable P. chrysogenum conidia for 2 weeks. Results: C57BL/6 mice inoculated with low numbers of conidia developed no significant lung inflammation or increased serum immunoglobulins. Mice sensitized to the protease extract and challenged with both protease extract and viable conidia produced significant increases in serum IgE and IgG1. Mice sensitized to and challenged with the protease extract developed significant eosinophilia and mucus hyperproduction as determined by bronchoalveolar lavage and histopathological examination of lung tissue. Conclusions: Mice did not develop allergic symptoms in response to challenge with low levels of P. chrysogenum conidia. Protease allergens from viable conidia induced specific allergic responses in mice, indicating the importance of P. chrysogenum conidia in allergic sensitization to the organism.

AB - Background: Previous evidence by our laboratory has shown that mice inoculated with viable Penicillium chrysogenum conidia or spores at levels comparable to those found in contaminated buildings induced spore antigen-specific allergic responses. We proposed that mice exposed to low levels of viable P. chrysogenum conidia would not develop allergic symptoms. We also hypothesized that the symptoms induced by high numbers of conidia were the result of sensitization to allergens released by the conidia. Methods: C57BL/6 and BALB/c mice were exposed to 1 × 102 viable P. chrysogenum conidia by intranasal instillation weekly for a period of 11 weeks. C57BL/6 mice were also sensitized to a viable P. chrysogenum conidia protease extract by intraperitoneal injections for a period of 6 weeks followed by intranasal challenge with protease extract, viable, or nonviable P. chrysogenum conidia for 2 weeks. Results: C57BL/6 mice inoculated with low numbers of conidia developed no significant lung inflammation or increased serum immunoglobulins. Mice sensitized to the protease extract and challenged with both protease extract and viable conidia produced significant increases in serum IgE and IgG1. Mice sensitized to and challenged with the protease extract developed significant eosinophilia and mucus hyperproduction as determined by bronchoalveolar lavage and histopathological examination of lung tissue. Conclusions: Mice did not develop allergic symptoms in response to challenge with low levels of P. chrysogenum conidia. Protease allergens from viable conidia induced specific allergic responses in mice, indicating the importance of P. chrysogenum conidia in allergic sensitization to the organism.

KW - Allergy

KW - Conidia

KW - Eosinophils

KW - IgE

KW - IgG1

KW - Mold

KW - Murine model

KW - Penicillium chrysogenum

KW - Sick building syndrome

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