Characterization of glutathione S-transferases of human cornea

Shivendra V. Singh, Thomas D. Hong, Satish Srivastava, Yogesh C. Awasthi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Two cationic (pIs 9·1 and 7·6) and one anionic (pI 4·4) forms of glutathione S-transferase have been purified to an apparent homogeneity from human cornea using glutathione-linked affinity chromatography and isoelectric focusing. The substrate specificities of the three enzyme forms are significantly different from each other. None of the three forms of human cornea glutathione S-transferase express glutathione peroxidase II activity. Immunological and structural studies reveal that human cornea enzymes have structural similarities with glutathione S-transferases of other human tissues.

Original languageEnglish (US)
Pages (from-to)431-437
Number of pages7
JournalExperimental Eye Research
Volume40
Issue number3
DOIs
StatePublished - 1985

Fingerprint

Glutathione Transferase
Cornea
Isoelectric Focusing
Enzymes
Substrate Specificity
Affinity Chromatography
Glutathione

Keywords

  • detoxication
  • glutathione peroxidase II
  • glutathione S-transferases
  • human cornea

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Singh, S. V., Hong, T. D., Srivastava, S., & Awasthi, Y. C. (1985). Characterization of glutathione S-transferases of human cornea. Experimental Eye Research, 40(3), 431-437. https://doi.org/10.1016/0014-4835(85)90155-1

Characterization of glutathione S-transferases of human cornea. / Singh, Shivendra V.; Hong, Thomas D.; Srivastava, Satish; Awasthi, Yogesh C.

In: Experimental Eye Research, Vol. 40, No. 3, 1985, p. 431-437.

Research output: Contribution to journalArticle

Singh, SV, Hong, TD, Srivastava, S & Awasthi, YC 1985, 'Characterization of glutathione S-transferases of human cornea', Experimental Eye Research, vol. 40, no. 3, pp. 431-437. https://doi.org/10.1016/0014-4835(85)90155-1
Singh, Shivendra V. ; Hong, Thomas D. ; Srivastava, Satish ; Awasthi, Yogesh C. / Characterization of glutathione S-transferases of human cornea. In: Experimental Eye Research. 1985 ; Vol. 40, No. 3. pp. 431-437.
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