Characterization of insulin, insulin-like growth factors I and II, and growth hormone receptors on human leukemic lymphoblasts

Phillip Lee, R. G. Rosenfeld, R. L. Hintz, S. D. Smith

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Receptors for insulin, insulin-like growth factors I and II (IGF-I and IGF-II), and human GH were studied in 6 T- and 12 B-lymphoblast cell lines isolated from patients with lymphoid malignancies. These cell lines have been maintained in continuous culture with stable chromosome, immunophenotype, and enzyme characteristics for an 8- to 21-month period. Four of 6 T-cell lines expressed IGF-I, but not insulin, receptors. One T-cell line bound only insulin, and 1 T-cell line bound both hormones. Converseley, 9 of 12 B-cell lines had insulin, but not IGF-I, receptors. Two of these lines bound both hormones, and 1 line did not bind either hormone. IGF-II binding was less than 1.5%/10 million cells for all lines and was less than 1% for 13 of the 18 lines. Specific binding of GH was undetectable in all cell lines. Time, temperature, and pH dependence of peptide binding were characterized for the T-cell IGF-I receptor and the B-cell insulin receptor and were consistent with previously described models for these receptors. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the cross-linked receptors revealed an apparent mol wt greater than 300K unreduced and a 130K binding subunit after dithiothreitol reduction for both the T-cell IGF-I and the B-cell insulin receptor. These cell lines thus provided a unique opportunity for the study of growth factor receptors in fully characterized clonal populations of human lymphoblasts. We conclude that 1) specific receptors for IGF-I and insulin are present on T- and B-lymphoblasts; 2) divergence of these receptors appears to be a characteristic of lymphoblasts, since T-cells preferentially expressed IGF-I receptors and B-cells expressed insulin receptors; and 3) IGF-II binding was relatively low, while specific GH binding was undetectable on both T- and B-lymphoblasts. These results suggest that insulin and IGF-I may play a role in lymphocyte differentiation and metabolism.

Original languageEnglish (US)
Pages (from-to)28-35
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume62
Issue number1
StatePublished - 1986
Externally publishedYes

Fingerprint

Somatotropin Receptors
Insulin-Like Growth Factor II
Insulin-Like Growth Factor I
T-cells
Cells
Insulin
Insulin Receptor
IGF Type 1 Receptor
Cell Line
T-Lymphocytes
B-Lymphocytes
Hormones
Lymphocytes
Growth Factor Receptors
delta-hGHR
Dithiothreitol
Chromosomes
Electrophoresis
Cell culture
Metabolism

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Characterization of insulin, insulin-like growth factors I and II, and growth hormone receptors on human leukemic lymphoblasts. / Lee, Phillip; Rosenfeld, R. G.; Hintz, R. L.; Smith, S. D.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 62, No. 1, 1986, p. 28-35.

Research output: Contribution to journalArticle

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abstract = "Receptors for insulin, insulin-like growth factors I and II (IGF-I and IGF-II), and human GH were studied in 6 T- and 12 B-lymphoblast cell lines isolated from patients with lymphoid malignancies. These cell lines have been maintained in continuous culture with stable chromosome, immunophenotype, and enzyme characteristics for an 8- to 21-month period. Four of 6 T-cell lines expressed IGF-I, but not insulin, receptors. One T-cell line bound only insulin, and 1 T-cell line bound both hormones. Converseley, 9 of 12 B-cell lines had insulin, but not IGF-I, receptors. Two of these lines bound both hormones, and 1 line did not bind either hormone. IGF-II binding was less than 1.5{\%}/10 million cells for all lines and was less than 1{\%} for 13 of the 18 lines. Specific binding of GH was undetectable in all cell lines. Time, temperature, and pH dependence of peptide binding were characterized for the T-cell IGF-I receptor and the B-cell insulin receptor and were consistent with previously described models for these receptors. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the cross-linked receptors revealed an apparent mol wt greater than 300K unreduced and a 130K binding subunit after dithiothreitol reduction for both the T-cell IGF-I and the B-cell insulin receptor. These cell lines thus provided a unique opportunity for the study of growth factor receptors in fully characterized clonal populations of human lymphoblasts. We conclude that 1) specific receptors for IGF-I and insulin are present on T- and B-lymphoblasts; 2) divergence of these receptors appears to be a characteristic of lymphoblasts, since T-cells preferentially expressed IGF-I receptors and B-cells expressed insulin receptors; and 3) IGF-II binding was relatively low, while specific GH binding was undetectable on both T- and B-lymphoblasts. These results suggest that insulin and IGF-I may play a role in lymphocyte differentiation and metabolism.",
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