Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E, NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody

Lauren C. Iacono-Connors, Jonathan F. Smith, Thomas G. Ksiazek, Cynthia L. Kelley, Connie S. Schmaljohn

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Hybridomas secreting monoclonal antibodies (MAb) to the tick-borne encephalitis (TBE) group virus. Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 14 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TBE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TBE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of any of the tick-borne flaviviruses.

Original languageEnglish (US)
Pages (from-to)125-136
Number of pages12
JournalVirus Research
Volume43
Issue number2
DOIs
StatePublished - Aug 1996
Externally publishedYes

Keywords

  • Hybridomas
  • Monoclonal antibodies
  • Tick-borne encephalitis

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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