Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E, NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody

Lauren C. Iacono-Connors, Jonathan F. Smith, Thomas Ksiazek, Cynthia L. Kelley, Connie S. Schmaljohn

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Hybridomas secreting monoclonal antibodies (MAb) to the tick-borne encephalitis (TBE) group virus. Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 14 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TBE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TBE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of any of the tick-borne flaviviruses.

Original languageEnglish (US)
Pages (from-to)125-136
Number of pages12
JournalVirus Research
Volume43
Issue number2
DOIs
StatePublished - Aug 1996
Externally publishedYes

Fingerprint

Tick-Borne Encephalitis Viruses
Epitopes
Monoclonal Antibodies
Antibodies
Arthropod Proteins
Tick-Borne Encephalitis
Flavivirus
Hybridomas
Proteins
Cell Culture Techniques
Viruses

Keywords

  • Hybridomas
  • Monoclonal antibodies
  • Tick-borne encephalitis

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E, NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody. / Iacono-Connors, Lauren C.; Smith, Jonathan F.; Ksiazek, Thomas; Kelley, Cynthia L.; Schmaljohn, Connie S.

In: Virus Research, Vol. 43, No. 2, 08.1996, p. 125-136.

Research output: Contribution to journalArticle

@article{091a2dca4ecb4e828a3e507a23423306,
title = "Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E, NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody",
abstract = "Hybridomas secreting monoclonal antibodies (MAb) to the tick-borne encephalitis (TBE) group virus. Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 14 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TBE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TBE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of any of the tick-borne flaviviruses.",
keywords = "Hybridomas, Monoclonal antibodies, Tick-borne encephalitis",
author = "Iacono-Connors, {Lauren C.} and Smith, {Jonathan F.} and Thomas Ksiazek and Kelley, {Cynthia L.} and Schmaljohn, {Connie S.}",
year = "1996",
month = "8",
doi = "10.1016/0168-1702(96)01325-1",
language = "English (US)",
volume = "43",
pages = "125--136",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Characterization of Langat virus antigenic determinants defined by monoclonal antibodies to E, NS1 and preM and identification of a protective, non-neutralizing preM-specific monoclonal antibody

AU - Iacono-Connors, Lauren C.

AU - Smith, Jonathan F.

AU - Ksiazek, Thomas

AU - Kelley, Cynthia L.

AU - Schmaljohn, Connie S.

PY - 1996/8

Y1 - 1996/8

N2 - Hybridomas secreting monoclonal antibodies (MAb) to the tick-borne encephalitis (TBE) group virus. Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 14 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TBE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TBE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of any of the tick-borne flaviviruses.

AB - Hybridomas secreting monoclonal antibodies (MAb) to the tick-borne encephalitis (TBE) group virus. Langat virus (LGTV), were prepared. Of more than 200 MAb screened, 19 antibodies, which cross-reacted with the etiologic agent of Central European encephalitis, were selected for further characterization. Of these MAb, 14 were specific for LGTV E glycoprotein, two for the NS1 protein, and three for preM protein. The two NS1-specific MAb and two of the E-specific MAb reacted with all six of the other TBE group viruses tested while the remainder of the E-specific MAb failed to recognize at least one of the viruses. None of the MAb neutralized LGTV in cell culture assays, but one of the preM-specific MAb protected weanling mice against a virulent LGTV challenge. Although protective antibodies to E and NS1 proteins of TBE viruses were reported, our data provided the first evidence for protection by a non-neutralizing antibody to the preM or M protein of any of the tick-borne flaviviruses.

KW - Hybridomas

KW - Monoclonal antibodies

KW - Tick-borne encephalitis

UR - http://www.scopus.com/inward/record.url?scp=0030221144&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030221144&partnerID=8YFLogxK

U2 - 10.1016/0168-1702(96)01325-1

DO - 10.1016/0168-1702(96)01325-1

M3 - Article

VL - 43

SP - 125

EP - 136

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 2

ER -