Mount Elgon Bat (MEB) virus, an isolate from a Rhinolophus hildebrandtii eloquens (K. Anderson) bat from Kenya, was examined by negative contrast and thin-section electron microscopy. It was found in infected mouse brain tissue to be bullet-shaped with structural details similar to other viruses of the Rhabdovirus group. Virus particles had a mean length of 226 nm and were 68 nm in diameter. The internal helically wound cylinder consisted of a single strand approximately 10 nm in diameter. In mouse brain, virus maturation occurred upon plasma membranes; viral matrix or inclusion material was in many cases located in the cytoplasm beneath sites of viral budding. The matrix consisted of convoluted filaments or strands. Complement-fixation, neutralization, and immunodiffusion tests comparing MEB virus with all the known Rhabdoviruses of animals failed to show any cross-reactivity. The described characteristics form the basis for considering MEB as an antigenically distinct member of the Rhabdovirus group.
ASJC Scopus subject areas