Characterization of multiple forms of the ah receptor

Recognition of a dioxin-responsive enhancer involves heteromer formation

Thomas A. Gasiewicz, Cornelis Elferink, E. C. Henry

Research output: Contribution to journalArticle

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Abstract

We have employed a combination of gel retardation, protein-DNA cross-linking, and protein-protein cross-linking techniques to further examine the 2,3,7,8-tetrachlorodibenzo-p-dioxin- (TCDD-) dependent changes in the Ah receptor that result in a DNA-binding conformation. Gel retardation analysis of DNA-Sepharose chromatographic fractions of rat hepatic cytosol indicated that TCDD-dependent and sequence-specific DNA binding coeluted with a 200-kDa form of the Ah receptor (peak 2) previously characterized as being multimeric and having high affinity for calf thymus DNA. The TCDD-bound, 100-kDa form of the receptor (peak 1) bound weakly to the DNA recognition motif. These results indicated that the DNA-binding form of the Ah receptor is a multimer. SDS-polyacrylamide gel electrophoresis of peak 2 cross-linked to a bromodeoxyuridine-substituted DNA recognition motif indicated that this form of the receptor present in rat hepatic cytosol is composed of at least two DNA-binding proteins of approximately 100 and 110 kDa. Using the chemical cross-linking agent dimethyl pimelimidate, we further established that the 100-kDa form of the receptor (peak 1) associates with a different protein to generate the receptor form (peak 2) that binds to the dioxin-responsive enhancer. Photoaffinity-labeling studies indicated that only the 100-kDa protein (peak 1), and not the 110-kDa protein, binds ligand. Together, these observations imply that the DNA-binding form of the Ah receptor exists as a heteromer.

Original languageEnglish (US)
Pages (from-to)2909-2916
Number of pages8
JournalBiochemistry
Volume30
Issue number11
StatePublished - 1991
Externally publishedYes

Fingerprint

Dioxins
DNA
Nucleotide Motifs
Proteins
Cytosol
Gels
Nucleic Acid Conformation
Rats
Liver
DNA-Binding Proteins
Bromodeoxyuridine
Sepharose
Polyacrylamide Gel Electrophoresis
Electrophoresis
Labeling
Ligands
Conformations
Polychlorinated Dibenzodioxins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of multiple forms of the ah receptor : Recognition of a dioxin-responsive enhancer involves heteromer formation. / Gasiewicz, Thomas A.; Elferink, Cornelis; Henry, E. C.

In: Biochemistry, Vol. 30, No. 11, 1991, p. 2909-2916.

Research output: Contribution to journalArticle

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