Characterization of Nodamura virus, an arthropod transmissible picornavirus

Frederick A. Murphy, William F. Scherer, Alyne K. Harrison, H. W. Dunne, G. William Gary

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Nodamura virus, an arthropod-transmissible virus which is resistant to ether and chloroform, was characterized by electron microscopic, physical, and additional immunological studies. Examination of thin sections of limb muscles of infected infant mice revealed extremely large numbers of virus particles (28 nm in diameter) dispersed and in crystalline array within sarcoplasm. Two kinds of viroplasmic inclusions and severe destruction of skeletal muscle cell architecture were associated with the presence of virus. Kupffer cells in the liver of these animals also contained large aggregates of virus particles. Negative contrast preparations contained virus particles 29 nm in diameter with cubic symmetry. The virus was morphologically indistinguishable from picornaviruses. The density of infectious virus was 1.34 g/ml; it was insensitive to pH 3.7, and its sensitivity to heat (50 °) was not stabilized by molar MgCl2. Since serum neutralization tests against 10 swine enteroviruses were negative, as were previously reported attempts at serological identification, Nodamura virus remains a picornavirus unrelated to any known virus, but with a demonstrated capacity for transmission by and multiplication in arthropods.

Original languageEnglish (US)
Pages (from-to)1008-1021
Number of pages14
JournalVirology
Volume40
Issue number4
DOIs
StatePublished - 1970

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Picornaviridae
Arthropods
Viruses
Virion
Neutralization Tests
Magnesium Chloride
Kupffer Cells
Enterovirus
Chloroform
Ether
Muscle Cells
Skeletal Muscle
Swine
Extremities
Hot Temperature
Electrons
Muscles
Liver
Serum

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Murphy, F. A., Scherer, W. F., Harrison, A. K., Dunne, H. W., & Gary, G. W. (1970). Characterization of Nodamura virus, an arthropod transmissible picornavirus. Virology, 40(4), 1008-1021. https://doi.org/10.1016/0042-6822(70)90147-9

Characterization of Nodamura virus, an arthropod transmissible picornavirus. / Murphy, Frederick A.; Scherer, William F.; Harrison, Alyne K.; Dunne, H. W.; Gary, G. William.

In: Virology, Vol. 40, No. 4, 1970, p. 1008-1021.

Research output: Contribution to journalArticle

Murphy, FA, Scherer, WF, Harrison, AK, Dunne, HW & Gary, GW 1970, 'Characterization of Nodamura virus, an arthropod transmissible picornavirus', Virology, vol. 40, no. 4, pp. 1008-1021. https://doi.org/10.1016/0042-6822(70)90147-9
Murphy, Frederick A. ; Scherer, William F. ; Harrison, Alyne K. ; Dunne, H. W. ; Gary, G. William. / Characterization of Nodamura virus, an arthropod transmissible picornavirus. In: Virology. 1970 ; Vol. 40, No. 4. pp. 1008-1021.
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