Characterization of paracrystalline inclusions in chinese hamster oocytes and early embryos

T. A. Parkening, A. F. Payer, R. L. Given

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Paracrystalline inclusions, readily visible with light microscopy, were found to be present in ovarian oocytes (beginning with unilaminar follicles), ovulated ova, and preimplantation embryos of the Chinese hamster. These inclusions appeared to be aggregates of finer filamentous structures visible only with electron microscopy. The use of various histochemical techniques suggested that the paracrystals were composed of protein with little or no lipid associated with them. Autoradiographic studies using 3H‐uridine and enzymatic studies did not support the hypothesis that the paracrystalline inclusions were composed of RNA masked by a crystalline protein lattice.

Original languageEnglish (US)
Pages (from-to)373-384
Number of pages12
JournalGamete Research
Volume12
Issue number4
DOIs
StatePublished - 1985

Keywords

  • Chinese hamster
  • RNA
  • oocytes
  • paracrystalline inclusions
  • preimplantation embryos
  • ultrastructure

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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