Characterization of peptide-YY release in response to intracolonic infusion of amino acids

Tao Zhang, Patricia L. Brubaker, James C. Thompson, George H. Greeley

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Peptide-YY-(1-36) [PYY-(1-36)] is an endocrine peptide that is found primarily in the mucosal layer of the colon. We reported previously that a direct intracolonic (IC) infusion of amino acids in dogs resulted in a robust release of PYY compared to other nutrients (fat, glucose, and protein). The objective of this study was to investigate the possible mechanisms underlying the release of PYY in response to IC infusion of amino acids. Intracolonic infusion of a mixture of tryptophan and phenylalanine (Trp+Phe; 100 mM; 200 ml/h) resulted in a significant release of PYY [integrated PYY release, 74.5 ± 14.0 ng (0-120 min)/ml], which was not affected by iv atropine, hexamethonium, or propranolol treatment. Intravenous infusion of Trp+Phe failed to release PYY [integrated PYY release, -0.6 ± 0.9 ng (0-120 min)/ml]. Intracolonic infusion of aromatic amino acids (Trp+Phe) was more potent in releasing PYY than aliphatic (leucine and glycine) and charged (arginine) amino acids. PYY release in response to IC infusion of the deamino and decarboxylated forms of Phe was significantly (P < 0.05) decreased [integrated PYY release, 17.5 ± 2.6 and 12.1 ± 2.8 ng (0-120 min)/ml, respectively] compared to that in response to intact Phe [integrated PYY release, 39.4 ± 4.3 ng (0-120 min)/ml]. These results suggest that 1) PYY release in response to IC administered amino acids is independent of ganglionic, cholinergic, and β-adrenergic transmission; 2) PYY release in response to IC amino acids is due to a direct contact of amino acids with the luminal pole of PYY cells in the colon and is not the result of a stimulatory action of amino acids via the general circulation; and 3) the amino and carboxylic groups of an amino acid as well as the ring structures of aromatic amino acids are structural requirements for PYY release.

Original languageEnglish (US)
Pages (from-to)553-557
Number of pages5
JournalEndocrinology
Volume132
Issue number2
StatePublished - Feb 1993

Fingerprint

Peptide YY
Amino Acids
Aromatic Amino Acids
Colon
Hexamethonium
Phenylalanine
Atropine
Intravenous Infusions
Propranolol
Leucine
Tryptophan
Adrenergic Agents
Glycine
Cholinergic Agents
Arginine
Fats
Dogs
Glucose
Food
Peptides

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Zhang, T., Brubaker, P. L., Thompson, J. C., & Greeley, G. H. (1993). Characterization of peptide-YY release in response to intracolonic infusion of amino acids. Endocrinology, 132(2), 553-557.

Characterization of peptide-YY release in response to intracolonic infusion of amino acids. / Zhang, Tao; Brubaker, Patricia L.; Thompson, James C.; Greeley, George H.

In: Endocrinology, Vol. 132, No. 2, 02.1993, p. 553-557.

Research output: Contribution to journalArticle

Zhang, T, Brubaker, PL, Thompson, JC & Greeley, GH 1993, 'Characterization of peptide-YY release in response to intracolonic infusion of amino acids', Endocrinology, vol. 132, no. 2, pp. 553-557.
Zhang T, Brubaker PL, Thompson JC, Greeley GH. Characterization of peptide-YY release in response to intracolonic infusion of amino acids. Endocrinology. 1993 Feb;132(2):553-557.
Zhang, Tao ; Brubaker, Patricia L. ; Thompson, James C. ; Greeley, George H. / Characterization of peptide-YY release in response to intracolonic infusion of amino acids. In: Endocrinology. 1993 ; Vol. 132, No. 2. pp. 553-557.
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abstract = "Peptide-YY-(1-36) [PYY-(1-36)] is an endocrine peptide that is found primarily in the mucosal layer of the colon. We reported previously that a direct intracolonic (IC) infusion of amino acids in dogs resulted in a robust release of PYY compared to other nutrients (fat, glucose, and protein). The objective of this study was to investigate the possible mechanisms underlying the release of PYY in response to IC infusion of amino acids. Intracolonic infusion of a mixture of tryptophan and phenylalanine (Trp+Phe; 100 mM; 200 ml/h) resulted in a significant release of PYY [integrated PYY release, 74.5 ± 14.0 ng (0-120 min)/ml], which was not affected by iv atropine, hexamethonium, or propranolol treatment. Intravenous infusion of Trp+Phe failed to release PYY [integrated PYY release, -0.6 ± 0.9 ng (0-120 min)/ml]. Intracolonic infusion of aromatic amino acids (Trp+Phe) was more potent in releasing PYY than aliphatic (leucine and glycine) and charged (arginine) amino acids. PYY release in response to IC infusion of the deamino and decarboxylated forms of Phe was significantly (P < 0.05) decreased [integrated PYY release, 17.5 ± 2.6 and 12.1 ± 2.8 ng (0-120 min)/ml, respectively] compared to that in response to intact Phe [integrated PYY release, 39.4 ± 4.3 ng (0-120 min)/ml]. These results suggest that 1) PYY release in response to IC administered amino acids is independent of ganglionic, cholinergic, and β-adrenergic transmission; 2) PYY release in response to IC amino acids is due to a direct contact of amino acids with the luminal pole of PYY cells in the colon and is not the result of a stimulatory action of amino acids via the general circulation; and 3) the amino and carboxylic groups of an amino acid as well as the ring structures of aromatic amino acids are structural requirements for PYY release.",
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