Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA

Robert B. Tesh; Bethany G. Bolling; Hilda Guzman; Vsevolod L. Popov; Ashley Wilson; Steven G. Widen; Thomas G. Wood; Peter J. Walker; Nikos Vasilakis

doi:10.3390/v12040390

Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA

Robert B. Tesh, Bethany G. Bolling, Hilda Guzman, Vsevolod L. Popov, Ashley Wilson, Steven G. Widen, Thomas G. Wood, Peter J. Walker, Nikos Vasilakis

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

Original language	English (US)
Article number	390
Journal	Viruses
Volume	12
Issue number	4
DOIs	https://doi.org/10.3390/v12040390
State	Published - 2020

Keywords

Birnaviridae
Entomobirnavirus
Mosquito viruses
Port Bolivar virus

ASJC Scopus subject areas

Infectious Diseases
Virology

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10.3390/v12040390

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title = "Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA",

abstract = "This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.",

keywords = "Birnaviridae, Entomobirnavirus, Mosquito viruses, Port Bolivar virus",

author = "Tesh, {Robert B.} and Bolling, {Bethany G.} and Hilda Guzman and Popov, {Vsevolod L.} and Ashley Wilson and Widen, {Steven G.} and Wood, {Thomas G.} and Walker, {Peter J.} and Nikos Vasilakis",

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year = "2020",

doi = "10.3390/v12040390",

language = "English (US)",

volume = "12",

journal = "Viruses",

issn = "1999-4915",

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AU - Tesh, Robert B.

AU - Bolling, Bethany G.

AU - Guzman, Hilda

AU - Popov, Vsevolod L.

AU - Wilson, Ashley

AU - Widen, Steven G.

AU - Wood, Thomas G.

AU - Walker, Peter J.

AU - Vasilakis, Nikos

PY - 2020

Y1 - 2020

N2 - This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

AB - This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

KW - Birnaviridae

KW - Entomobirnavirus

KW - Mosquito viruses

KW - Port Bolivar virus

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Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA

Abstract

Keywords

ASJC Scopus subject areas

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