Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA

Robert B. Tesh, Bethany G. Bolling, Hilda Guzman, Vsevolod L. Popov, Ashley Wilson, Steven G. Widen, Thomas Wood, Peter J. Walker, Nikos Vasilakis

Research output: Contribution to journalArticle

Abstract

This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

Original languageEnglish (US)
Article number390
JournalViruses
Volume12
Issue number4
DOIs
StatePublished - Jan 1 2020

Keywords

  • Birnaviridae
  • Entomobirnavirus
  • Mosquito viruses
  • Port Bolivar virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Fingerprint Dive into the research topics of 'Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA'. Together they form a unique fingerprint.

  • Cite this