Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA

Robert B. Tesh; Bethany G. Bolling; Hilda Guzman; Vsevolod L. Popov; Ashley Wilson; Steven G. Widen; Thomas G. Wood; Peter J. Walker; Nikos Vasilakis

doi:10.3390/v12040390

Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA

Robert B. Tesh
, Bethany G. Bolling
, Hilda Guzman
, Vsevolod L. Popov
, Ashley Wilson
, Steven G. Widen
, Thomas G. Wood
, Peter J. Walker
, Nikos Vasilakis

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

Original language	English (US)
Article number	390
Journal	Viruses
Volume	12
Issue number	4
DOIs	https://doi.org/10.3390/v12040390
State	Published - 2020

Keywords

Birnaviridae
Entomobirnavirus
Mosquito viruses
Port Bolivar virus

ASJC Scopus subject areas

Infectious Diseases
Virology

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10.3390/v12040390

Cite this

@article{2720ba951ef645b8b3d31f63392b6a30,

title = "Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA",

abstract = "This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.",

keywords = "Birnaviridae, Entomobirnavirus, Mosquito viruses, Port Bolivar virus",

author = "Tesh, \{Robert B.\} and Bolling, \{Bethany G.\} and Hilda Guzman and Popov, \{Vsevolod L.\} and Ashley Wilson and Widen, \{Steven G.\} and Wood, \{Thomas G.\} and Walker, \{Peter J.\} and Nikos Vasilakis",

note = "Publisher Copyright: {\textcopyright} 2020 by the authors.",

year = "2020",

doi = "10.3390/v12040390",

language = "English (US)",

volume = "12",

journal = "Viruses",

issn = "1999-4915",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "4",

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TY - JOUR

T1 - Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA

AU - Tesh, Robert B.

AU - Bolling, Bethany G.

AU - Guzman, Hilda

AU - Popov, Vsevolod L.

AU - Wilson, Ashley

AU - Widen, Steven G.

AU - Wood, Thomas G.

AU - Walker, Peter J.

AU - Vasilakis, Nikos

PY - 2020

Y1 - 2020

N2 - This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

AB - This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

KW - Birnaviridae

KW - Entomobirnavirus

KW - Mosquito viruses

KW - Port Bolivar virus

UR - https://www.scopus.com/pages/publications/85082818306

UR - https://www.scopus.com/pages/publications/85082818306#tab=citedBy

U2 - 10.3390/v12040390

DO - 10.3390/v12040390

M3 - Article

C2 - 32244531

AN - SCOPUS:85082818306

SN - 1999-4915

VL - 12

JO - Viruses

JF - Viruses

IS - 4

M1 - 390

ER -

Characterization of port bolivar virus, a novel entomobirnavirus (birnaviridae) isolated from mosquitoes collected in east Texas, USA

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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