Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil

Luciane Daniele Cardoso; Renata Nascimento Freitas; Cláudio Lísias Mafra; Cristiane Vilas Boas Neves; Fátima Cristina Bacellar Figueira; Marcelo Bahia Labruna; Solange M. Gennari; David Hughes Walker; Márcio Antônio Moreira Galvão

doi:10.1590/s0102-311x2006000300004

Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil

Translated title of the contribution: Characterization of Rickettsia spp. circulating in a silent peri-urban focus for Brazilian spotted fever in Caratinga, Minas Gerais, Brazil

Luciane Daniele Cardoso, Renata Nascimento Freitas, Cláudio Lísias Mafra, Cristiane Vilas Boas Neves, Fátima Cristina Bacellar Figueira, Marcelo Bahia Labruna, Solange M. Gennari, David Hughes Walker, Márcio Antônio Moreira Galvão

Pathology

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The present study was intended to characterize Rickettsia spp. circulating in arthropod vectors in Caratinga, Minas Gerais, Brazil, by PCR and to investigate the presence of antibodies against the spotted fever Rickettsiae group (SFRG) in dogs and horses. 2,610 arthropods were collected and taxonomically identified. DNA samples obtained from these vectors were submitted to PCR and cycle-sequenced. Ctenocephalides and Amblyomma cajennense showed sequences presenting 100.0% homology with R. felis. A sequence obtained from Rhipicephalus sanguineus showed 99.0% homology with R. felis, and a sequence from A. cajennense showed 97.0% homology with R. honei and R. rickettsii. Canine (73) and equine (18) serum samples were tested by indirect fluorescent antibody (IFA) using R. rickettsii antigen. Only three of the equine sera tested (17.0%) had positive antibody titers. Molecular detection of rickettsiae species potentially pathogenic to humans in arthropod vectors and the presence of seroreactivity to SFRG in horses show the risk of transmission of rickettsiosis in this area and the need to maintain continuous epidemiological surveillance for rickettsial diseases.

Translated title of the contribution	Characterization of Rickettsia spp. circulating in a silent peri-urban focus for Brazilian spotted fever in Caratinga, Minas Gerais, Brazil
Original language	Portuguese
Pages (from-to)	495-501
Number of pages	7
Journal	Cadernos de Saude Publica
Volume	22
Issue number	3
DOIs	https://doi.org/10.1590/s0102-311x2006000300004
State	Published - Mar 2006

Keywords

Arthropod Vectors
Rickettsia
Rickettsia Infectious
Spotted Fever

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health

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10.1590/s0102-311x2006000300004

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Cardoso, L. D., Freitas, R. N., Mafra, C. L., Neves, C. V. B., Bacellar Figueira, F. C., Labruna, M. B., Gennari, S. M., Walker, D. H., & Galvão, M. A. M. (2006). Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil. Cadernos de Saude Publica, 22(3), 495-501. https://doi.org/10.1590/s0102-311x2006000300004

Cardoso, LD, Freitas, RN, Mafra, CL, Neves, CVB, Bacellar Figueira, FC, Labruna, MB, Gennari, SM, Walker, DH & Galvão, MAM 2006, 'Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil', Cadernos de Saude Publica, vol. 22, no. 3, pp. 495-501. https://doi.org/10.1590/s0102-311x2006000300004

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AU - Cardoso, Luciane Daniele

AU - Freitas, Renata Nascimento

AU - Mafra, Cláudio Lísias

AU - Neves, Cristiane Vilas Boas

AU - Bacellar Figueira, Fátima Cristina

AU - Labruna, Marcelo Bahia

AU - Gennari, Solange M.

AU - Walker, David Hughes

AU - Galvão, Márcio Antônio Moreira

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AB - The present study was intended to characterize Rickettsia spp. circulating in arthropod vectors in Caratinga, Minas Gerais, Brazil, by PCR and to investigate the presence of antibodies against the spotted fever Rickettsiae group (SFRG) in dogs and horses. 2,610 arthropods were collected and taxonomically identified. DNA samples obtained from these vectors were submitted to PCR and cycle-sequenced. Ctenocephalides and Amblyomma cajennense showed sequences presenting 100.0% homology with R. felis. A sequence obtained from Rhipicephalus sanguineus showed 99.0% homology with R. felis, and a sequence from A. cajennense showed 97.0% homology with R. honei and R. rickettsii. Canine (73) and equine (18) serum samples were tested by indirect fluorescent antibody (IFA) using R. rickettsii antigen. Only three of the equine sera tested (17.0%) had positive antibody titers. Molecular detection of rickettsiae species potentially pathogenic to humans in arthropod vectors and the presence of seroreactivity to SFRG in horses show the risk of transmission of rickettsiosis in this area and the need to maintain continuous epidemiological surveillance for rickettsial diseases.

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KW - Spotted Fever

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Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil

Abstract

Keywords

ASJC Scopus subject areas

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