Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods

Claudia Silva, Laura Betancor, Coralith García, Lizeth Astocondor, Noemí Hinostroza, Julieta Bisio, Javier Rivera, Lucía Perezgasga, Victoria Pérez Escanda, Lucía Yim, Jan Jacobs, José A. Chabalgoity, Francisco García del Portillo, José L. Puente

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, different molecular typing tools were applied to characterize 95 Salmonella enterica blood isolates collected between 2008 and 2013 from patients at nine public hospitals in Lima, Peru. Combined results of multiplex PCR serotyping, two- and seven-loci multilocus sequence typing (MLST) schemes, serotyping, IS200 amplification and RAPD fingerprints, showed that these infections were caused by eight different serovars: Enteritidis, Typhimurium, Typhi, Choleraesuis, Dublin, Paratyphi A, Paratyphi B and Infantis. Among these, Enteritidis, Typhimurium and Typhi were the most prevalent, representing 45, 36 and 11% of the isolates, respectively. Most isolates (74%) were not resistant to ten primarily used antimicrobial drugs; however, 37% of the strains showed intermediate susceptibility to ciprofloxacin (ISC). Antimicrobial resistance integrons were carried by one Dublin (dfra1 and aadA1) and two Infantis (aadA1) isolates. The two Infantis isolates were multidrug resistant and harbored a large megaplasmid. Amplification of spvC and spvRA regions showed that all Enteritidis (n = 42), Typhimurium (n = 34), Choleraesuis (n = 3) and Dublin (n = 1) isolates carried the Salmonella virulence plasmid (pSV). We conclude that the classic serotyping method can be substituted by the multiplex PCR and, when necessary, sequencing of only one or two loci of the MLST scheme is a valuable tool to confirm the results. The effectiveness and feasibility of different typing tools is discussed.

Original languageEnglish (US)
Article numbere0189946
JournalPLoS One
Volume12
Issue number12
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

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Serotyping
bacteremia
Peru
Salmonella
Salmonella enterica
Bacteremia
Multilocus Sequence Typing
Multiplex Polymerase Chain Reaction
loci
Amplification
plasmids
Integrons
Molecular Typing
Public Hospitals
ciprofloxacin
Dermatoglyphics
Ciprofloxacin
antibiotic resistance
Virulence
serotypes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Silva, C., Betancor, L., García, C., Astocondor, L., Hinostroza, N., Bisio, J., ... Puente, J. L. (2017). Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods. PLoS One, 12(12), [e0189946]. https://doi.org/10.1371/journal.pone.0189946

Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods. / Silva, Claudia; Betancor, Laura; García, Coralith; Astocondor, Lizeth; Hinostroza, Noemí; Bisio, Julieta; Rivera, Javier; Perezgasga, Lucía; Escanda, Victoria Pérez; Yim, Lucía; Jacobs, Jan; Chabalgoity, José A.; Portillo, Francisco García del; Puente, José L.

In: PLoS One, Vol. 12, No. 12, e0189946, 01.12.2017.

Research output: Contribution to journalArticle

Silva, C, Betancor, L, García, C, Astocondor, L, Hinostroza, N, Bisio, J, Rivera, J, Perezgasga, L, Escanda, VP, Yim, L, Jacobs, J, Chabalgoity, JA, Portillo, FGD & Puente, JL 2017, 'Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods', PLoS One, vol. 12, no. 12, e0189946. https://doi.org/10.1371/journal.pone.0189946
Silva, Claudia ; Betancor, Laura ; García, Coralith ; Astocondor, Lizeth ; Hinostroza, Noemí ; Bisio, Julieta ; Rivera, Javier ; Perezgasga, Lucía ; Escanda, Victoria Pérez ; Yim, Lucía ; Jacobs, Jan ; Chabalgoity, José A. ; Portillo, Francisco García del ; Puente, José L. / Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods. In: PLoS One. 2017 ; Vol. 12, No. 12.
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