Characterization of small plaque mutants of mouse hepatitis virus, JHM strain.

Shinji Makino, F. Taguchi, M. Hayami, K. Fujiwara

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Two small plaque mutants designated as 1a and 2c were isolated from DBT cells persistently infected with the JHM strain of mouse hepatitis virus. Unlike the wild type JHM, these two mutant viruses grew more slowly with no prominent cell fusion. The buoyant densities of the mutants were slightly lower and 2c was revealed to have fewer peplomers than JHM by electron microscopy. The purified JHM contained five polypeptides with molecular weights (M.W.) of 260,000, 105,000 (GP105), 65,000, 60,000 (P60), and 23,000 (GP23). In addition to two polypeptides, P60 and GP23, which were common to JHM and the mutants, 1a was found to contain three other specific polypeptides with M.W. of 180,000 (GP180), 110,000, and 95,000 (GP95), while 2c had GP180, GP105, GP95, and one with a M.W. of 175,000. All of these polypeptides were shown to be glycosylated except for P60. After bromelain treatment, all these viruses lost the peplomers and contained P60 and another new 18,000 dalton polypeptide.

Original languageEnglish (US)
Pages (from-to)445-454
Number of pages10
JournalMicrobiology and Immunology
Volume27
Issue number5
StatePublished - 1983
Externally publishedYes

Fingerprint

Murine hepatitis virus
Peptides
Molecular Weight
Bromelains
Viruses
Cell Fusion
Electron Microscopy

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Characterization of small plaque mutants of mouse hepatitis virus, JHM strain. / Makino, Shinji; Taguchi, F.; Hayami, M.; Fujiwara, K.

In: Microbiology and Immunology, Vol. 27, No. 5, 1983, p. 445-454.

Research output: Contribution to journalArticle

Makino, S, Taguchi, F, Hayami, M & Fujiwara, K 1983, 'Characterization of small plaque mutants of mouse hepatitis virus, JHM strain.', Microbiology and Immunology, vol. 27, no. 5, pp. 445-454.
Makino, Shinji ; Taguchi, F. ; Hayami, M. ; Fujiwara, K. / Characterization of small plaque mutants of mouse hepatitis virus, JHM strain. In: Microbiology and Immunology. 1983 ; Vol. 27, No. 5. pp. 445-454.
@article{51486622e5af4a5e80a46687b06d3289,
title = "Characterization of small plaque mutants of mouse hepatitis virus, JHM strain.",
abstract = "Two small plaque mutants designated as 1a and 2c were isolated from DBT cells persistently infected with the JHM strain of mouse hepatitis virus. Unlike the wild type JHM, these two mutant viruses grew more slowly with no prominent cell fusion. The buoyant densities of the mutants were slightly lower and 2c was revealed to have fewer peplomers than JHM by electron microscopy. The purified JHM contained five polypeptides with molecular weights (M.W.) of 260,000, 105,000 (GP105), 65,000, 60,000 (P60), and 23,000 (GP23). In addition to two polypeptides, P60 and GP23, which were common to JHM and the mutants, 1a was found to contain three other specific polypeptides with M.W. of 180,000 (GP180), 110,000, and 95,000 (GP95), while 2c had GP180, GP105, GP95, and one with a M.W. of 175,000. All of these polypeptides were shown to be glycosylated except for P60. After bromelain treatment, all these viruses lost the peplomers and contained P60 and another new 18,000 dalton polypeptide.",
author = "Shinji Makino and F. Taguchi and M. Hayami and K. Fujiwara",
year = "1983",
language = "English (US)",
volume = "27",
pages = "445--454",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "5",

}

TY - JOUR

T1 - Characterization of small plaque mutants of mouse hepatitis virus, JHM strain.

AU - Makino, Shinji

AU - Taguchi, F.

AU - Hayami, M.

AU - Fujiwara, K.

PY - 1983

Y1 - 1983

N2 - Two small plaque mutants designated as 1a and 2c were isolated from DBT cells persistently infected with the JHM strain of mouse hepatitis virus. Unlike the wild type JHM, these two mutant viruses grew more slowly with no prominent cell fusion. The buoyant densities of the mutants were slightly lower and 2c was revealed to have fewer peplomers than JHM by electron microscopy. The purified JHM contained five polypeptides with molecular weights (M.W.) of 260,000, 105,000 (GP105), 65,000, 60,000 (P60), and 23,000 (GP23). In addition to two polypeptides, P60 and GP23, which were common to JHM and the mutants, 1a was found to contain three other specific polypeptides with M.W. of 180,000 (GP180), 110,000, and 95,000 (GP95), while 2c had GP180, GP105, GP95, and one with a M.W. of 175,000. All of these polypeptides were shown to be glycosylated except for P60. After bromelain treatment, all these viruses lost the peplomers and contained P60 and another new 18,000 dalton polypeptide.

AB - Two small plaque mutants designated as 1a and 2c were isolated from DBT cells persistently infected with the JHM strain of mouse hepatitis virus. Unlike the wild type JHM, these two mutant viruses grew more slowly with no prominent cell fusion. The buoyant densities of the mutants were slightly lower and 2c was revealed to have fewer peplomers than JHM by electron microscopy. The purified JHM contained five polypeptides with molecular weights (M.W.) of 260,000, 105,000 (GP105), 65,000, 60,000 (P60), and 23,000 (GP23). In addition to two polypeptides, P60 and GP23, which were common to JHM and the mutants, 1a was found to contain three other specific polypeptides with M.W. of 180,000 (GP180), 110,000, and 95,000 (GP95), while 2c had GP180, GP105, GP95, and one with a M.W. of 175,000. All of these polypeptides were shown to be glycosylated except for P60. After bromelain treatment, all these viruses lost the peplomers and contained P60 and another new 18,000 dalton polypeptide.

UR - http://www.scopus.com/inward/record.url?scp=0020650813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020650813&partnerID=8YFLogxK

M3 - Article

C2 - 6312277

AN - SCOPUS:0020650813

VL - 27

SP - 445

EP - 454

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 5

ER -