Characterization of the Bhanja serogroup viruses (bunyaviridae): A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses

Keita Matsuno, Keita Weisend, Amelia P A Travassos da Rosa, Sarah L. Anzick, Eric Dahlstrom, Stephen F. Porcella, David W. Dorward, Xue Jie Yu, Robert B. Tesh, Hideki Ebihara

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Bhanja virus (BHAV) and its antigenically close relatives Forecariah virus (FORV), Kismayo virus (KISV), and Palma virus (PALV) are thought to be members of the family Bunyaviridae, but they have not been assigned to a genus or species. Despite their broad geographical distribution and reports that BHAV causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the Bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemical information for the virus proteins. In order to better define the molecular characteristics of this group, we determined the full-length sequences of the L, M, and S genome segments of multiple isolates of BHAV as well as FORV and PALV. The genome structures of these Bhanja viruses are similar to those of viruses belonging to the genus Phlebovirus. Functional domains and amino acid motifs in the viral proteins that are conserved among other known phleboviruses were also identified in proteins of the BHAV group. Phylogenetic and serological analyses revealed that the BHAVs are most closely related to the novel emerging tick-borne phleboviruses severe fever with thrombocytopenia syndrome virus and Heartland virus, which have recently been implicated as causing severe acute febrile illnesses associated with thrombocytopenia in humans in China and the United States. Our results indicate that the Bhanja serogroup viruses constitute a single novel species in the genus Phlebovirus. The results of this study should facilitate epidemiological surveillance for other, similar tick-borne phleboviruses that may represent unrecognized causes of febrile illness in humans.

Original languageEnglish (US)
Pages (from-to)3719-3728
Number of pages10
JournalJournal of Virology
Volume87
Issue number7
DOIs
StatePublished - Apr 2013

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Phlebovirus
Bunyaviridae
Ticks
ticks
serotypes
Viruses
viruses
fever
Fever
Serogroup
thrombocytopenia
Arecaceae
Thrombocytopenia
Genome
amino acid motifs
Viral Structures

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Characterization of the Bhanja serogroup viruses (bunyaviridae) : A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses. / Matsuno, Keita; Weisend, Keita; Travassos da Rosa, Amelia P A; Anzick, Sarah L.; Dahlstrom, Eric; Porcella, Stephen F.; Dorward, David W.; Yu, Xue Jie; Tesh, Robert B.; Ebihara, Hideki.

In: Journal of Virology, Vol. 87, No. 7, 04.2013, p. 3719-3728.

Research output: Contribution to journalArticle

Matsuno, K, Weisend, K, Travassos da Rosa, APA, Anzick, SL, Dahlstrom, E, Porcella, SF, Dorward, DW, Yu, XJ, Tesh, RB & Ebihara, H 2013, 'Characterization of the Bhanja serogroup viruses (bunyaviridae): A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses', Journal of Virology, vol. 87, no. 7, pp. 3719-3728. https://doi.org/10.1128/JVI.02845-12
Matsuno, Keita ; Weisend, Keita ; Travassos da Rosa, Amelia P A ; Anzick, Sarah L. ; Dahlstrom, Eric ; Porcella, Stephen F. ; Dorward, David W. ; Yu, Xue Jie ; Tesh, Robert B. ; Ebihara, Hideki. / Characterization of the Bhanja serogroup viruses (bunyaviridae) : A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses. In: Journal of Virology. 2013 ; Vol. 87, No. 7. pp. 3719-3728.
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abstract = "Bhanja virus (BHAV) and its antigenically close relatives Forecariah virus (FORV), Kismayo virus (KISV), and Palma virus (PALV) are thought to be members of the family Bunyaviridae, but they have not been assigned to a genus or species. Despite their broad geographical distribution and reports that BHAV causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the Bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemical information for the virus proteins. In order to better define the molecular characteristics of this group, we determined the full-length sequences of the L, M, and S genome segments of multiple isolates of BHAV as well as FORV and PALV. The genome structures of these Bhanja viruses are similar to those of viruses belonging to the genus Phlebovirus. Functional domains and amino acid motifs in the viral proteins that are conserved among other known phleboviruses were also identified in proteins of the BHAV group. Phylogenetic and serological analyses revealed that the BHAVs are most closely related to the novel emerging tick-borne phleboviruses severe fever with thrombocytopenia syndrome virus and Heartland virus, which have recently been implicated as causing severe acute febrile illnesses associated with thrombocytopenia in humans in China and the United States. Our results indicate that the Bhanja serogroup viruses constitute a single novel species in the genus Phlebovirus. The results of this study should facilitate epidemiological surveillance for other, similar tick-borne phleboviruses that may represent unrecognized causes of febrile illness in humans.",
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