Characterization of the bujaru, frijoles and tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil

Joaquim Pinto Nunes-Neto, William Marciel De Souza, Gustavo Olszanski Acrani, Marilia Farignoli Romeiro, Marcíliojorge Fumagalli, Luiz Carlos Vieira, Daniele Barbosa De Almeida Medeiros, Juliana Abreu Lima, Clayton Pereira Silva De Lima, Jedson Ferreira Cardoso, Luiz Tadeu Moraes Figueiredo, Sandro Patroca Da Silva, Robert Tesh, Márcio Roberto Teixeira Nunes, Pedro Fernando Da Costa Vasconcelos

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The genus Phlebovirus includes the sandfly fever viruses and tick-transmitted uukuviruses. Sandfly fever group viruses have been isolated from various vertebrate species and from phlebotomines and occasionally alternative arthropods, e.g. mosquitoes, or ceratopogonids of the genus Culicoides. Uukuniemi serogroup viruses have been isolated from various vertebrate species and from ticks. Despite the public health importance of some viruses of the genus, the genomic diversity of phleboviruses that could be incriminated as causative of human or veterinary diseases remains underestimated. Here we describe the nearly complete sequences and genomic characterization of two phleboviruses belonging to the Bujaru antigenic complex: the prototype species and the Munguba virus. Furthermore, six previously unclassified phleboviruses isolated in Brazil were also sequenced and characterized: Ambe, Anhanga, Joa, Uriurana, Urucuri and Tapara viruses. The results of the phylogenetic analysis indicated that these viruses group with viruses of three antigenic complexes (Bujaru, Tapara and frijoles clades), with two unclassified phleboviruses. We also performed genomic reassortment analysis and confirmed that there were no events for the viruses described in this study, but we found a new potential reassortment in Medjerda Valley virus, which contains S and L segments of Arbia virus, and probably a unique M segment, both viruses circulate in the same geographic region, indicating these two isolates represent two distinct viruses. This study provides insights into the genetic diversity, classification and evolution of phleboviruses.

Original languageEnglish (US)
Pages (from-to)585-594
Number of pages10
JournalJournal of General Virology
Volume98
Issue number4
DOIs
StatePublished - Apr 2017

Keywords

  • Brazilian amazon
  • Genetic characterization
  • Phleboviruses
  • Sandfly fever group
  • Ungrouped phlebovirus

ASJC Scopus subject areas

  • Virology

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    Nunes-Neto, J. P., De Souza, W. M., Acrani, G. O., Romeiro, M. F., Fumagalli, M., Vieira, L. C., De Almeida Medeiros, D. B., Lima, J. A., De Lima, C. P. S., Cardoso, J. F., Figueiredo, L. T. M., Da Silva, S. P., Tesh, R., Nunes, M. R. T., & Da Costa Vasconcelos, P. F. (2017). Characterization of the bujaru, frijoles and tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil. Journal of General Virology, 98(4), 585-594. https://doi.org/10.1099/jgv.0.000724