Characterization of the coronavirus M protein and nucleocapsid interaction in infected cells

K. Narayanan, A. Maeda, J. Maeda, Shinji Makino

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Coronavirus contains three envelope proteins, M, E and S, and a nucleocapsid, which consists of genomic RNA and N protein, within the viral envelope. We studied the macromolecular interactions involved in coronavirus assembly in cells infected with a murine coronavirus, mouse hepatitis virus (MHV). Coimmunoprecipitation analyses demonstrated an interaction between N protein and M protein in infected cells. Pulse-labeling experiments showed that newly synthesized, unglycosylated M protein interacted with N protein in a pre-Golgi compartment, which is part of the MHV budding site. Coimmunoprecipitation analyses further revealed that M protein interacted with only genomic-length MHV mRNA, mRNA 1, while N protein interacted with all MHV mRNAs. These data indicated that M protein interacted with the nucleocapsid, consisting of N protein and mRNA 1, in infected cells. The M protein-nucleocapsid interaction occurred in the absence of S and E proteins. Intracellular M protein-N protein interaction was maintained after removal of viral RNAs by RNase treatment. However, the M protein-N protein interaction did not occur in cells coexpressing M protein and N protein alone. These data indicated that while the M protein-N protein interaction, which is independent of viral RNA, occurred in the M protein-nucleocapsid complex, some MHV function(s) was necessary for the initiation of M protein-nucleocapsid interaction. The M protein-nucleocapsid interaction, which occurred near or at the MHV budding site, most probably represented the process of specific packaging of the MHV genome into MHV particles.

Original languageEnglish (US)
Pages (from-to)8127-8134
Number of pages8
JournalJournal of Virology
Volume74
Issue number17
DOIs
StatePublished - 2000

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Nucleocapsid
nucleocapsid
Coronavirinae
Murine hepatitis virus
Proteins
proteins
Nucleocapsid Proteins
cells
nucleocapsid proteins
Coronavirus
Virus Release
Messenger RNA
Coronavirus M protein
Viral RNA
RNA
Murine coronavirus
Viral Envelope Proteins
genomics
Protein S

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of the coronavirus M protein and nucleocapsid interaction in infected cells. / Narayanan, K.; Maeda, A.; Maeda, J.; Makino, Shinji.

In: Journal of Virology, Vol. 74, No. 17, 2000, p. 8127-8134.

Research output: Contribution to journalArticle

Narayanan, K. ; Maeda, A. ; Maeda, J. ; Makino, Shinji. / Characterization of the coronavirus M protein and nucleocapsid interaction in infected cells. In: Journal of Virology. 2000 ; Vol. 74, No. 17. pp. 8127-8134.
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