Characterization of the direct physical interaction of nc886, a cellular non-coding RNA, and PKR

Sung Ho Jeon, Kwanbok Lee, Kwang Soo Lee, Nawapol Kunkeaw, Betty H. Johnson, Luis Marcelo F Holthauzen, Bin Gong, Chanvit Leelayuwat, Yong Sun Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have recently shown that nc886 (pre-miR-886 or vtRNA2-1) is not a genuine microRNA precursor nor a vault RNA, but a novel type of non-coding RNA that represses PKR, a double-stranded RNA (dsRNA) dependent kinase. Here we have characterized their direct physical association. PKR's two RNA binding domains form a specific and stable complex with nc886's central portion, without any preference to its 5′-end structure. By binding to PKR with a comparable affinity, nc886 competes with dsRNA and attenuates PKR activation by dsRNA. Our data suggest that nc886 sets a threshold for PKR activation so that it occurs only during genuine viral infection but not by a minute level of fortuitous cellular dsRNA.

Original languageEnglish (US)
Pages (from-to)3477-3484
Number of pages8
JournalFEBS Letters
Volume586
Issue number19
DOIs
StatePublished - Sep 21 2012

Fingerprint

Untranslated RNA
Double-Stranded RNA
Chemical activation
RNA
Virus Diseases
MicroRNAs
Phosphotransferases
Association reactions

Keywords

  • Double-stranded RNA
  • nc886
  • PKR
  • Protein-RNA interaction
  • Vault RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Jeon, S. H., Lee, K., Lee, K. S., Kunkeaw, N., Johnson, B. H., Holthauzen, L. M. F., ... Lee, Y. S. (2012). Characterization of the direct physical interaction of nc886, a cellular non-coding RNA, and PKR. FEBS Letters, 586(19), 3477-3484. https://doi.org/10.1016/j.febslet.2012.07.076

Characterization of the direct physical interaction of nc886, a cellular non-coding RNA, and PKR. / Jeon, Sung Ho; Lee, Kwanbok; Lee, Kwang Soo; Kunkeaw, Nawapol; Johnson, Betty H.; Holthauzen, Luis Marcelo F; Gong, Bin; Leelayuwat, Chanvit; Lee, Yong Sun.

In: FEBS Letters, Vol. 586, No. 19, 21.09.2012, p. 3477-3484.

Research output: Contribution to journalArticle

Jeon, SH, Lee, K, Lee, KS, Kunkeaw, N, Johnson, BH, Holthauzen, LMF, Gong, B, Leelayuwat, C & Lee, YS 2012, 'Characterization of the direct physical interaction of nc886, a cellular non-coding RNA, and PKR', FEBS Letters, vol. 586, no. 19, pp. 3477-3484. https://doi.org/10.1016/j.febslet.2012.07.076
Jeon SH, Lee K, Lee KS, Kunkeaw N, Johnson BH, Holthauzen LMF et al. Characterization of the direct physical interaction of nc886, a cellular non-coding RNA, and PKR. FEBS Letters. 2012 Sep 21;586(19):3477-3484. https://doi.org/10.1016/j.febslet.2012.07.076
Jeon, Sung Ho ; Lee, Kwanbok ; Lee, Kwang Soo ; Kunkeaw, Nawapol ; Johnson, Betty H. ; Holthauzen, Luis Marcelo F ; Gong, Bin ; Leelayuwat, Chanvit ; Lee, Yong Sun. / Characterization of the direct physical interaction of nc886, a cellular non-coding RNA, and PKR. In: FEBS Letters. 2012 ; Vol. 586, No. 19. pp. 3477-3484.
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