Characterization of the direct physical interaction of nc886, a cellular non-coding RNA, and PKR

Sung Ho Jeon, Kwanbok Lee, Kwang Soo Lee, Nawapol Kunkeaw, Betty H. Johnson, Luis Marcelo F. Holthauzen, Bin Gong, Chanvit Leelayuwat, Yong Sun Lee

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

We have recently shown that nc886 (pre-miR-886 or vtRNA2-1) is not a genuine microRNA precursor nor a vault RNA, but a novel type of non-coding RNA that represses PKR, a double-stranded RNA (dsRNA) dependent kinase. Here we have characterized their direct physical association. PKR's two RNA binding domains form a specific and stable complex with nc886's central portion, without any preference to its 5′-end structure. By binding to PKR with a comparable affinity, nc886 competes with dsRNA and attenuates PKR activation by dsRNA. Our data suggest that nc886 sets a threshold for PKR activation so that it occurs only during genuine viral infection but not by a minute level of fortuitous cellular dsRNA.

Original languageEnglish (US)
Pages (from-to)3477-3484
Number of pages8
JournalFEBS Letters
Volume586
Issue number19
DOIs
StatePublished - Sep 21 2012

Keywords

  • Double-stranded RNA
  • PKR
  • Protein-RNA interaction
  • Vault RNA
  • nc886

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Jeon, S. H., Lee, K., Lee, K. S., Kunkeaw, N., Johnson, B. H., Holthauzen, L. M. F., Gong, B., Leelayuwat, C., & Lee, Y. S. (2012). Characterization of the direct physical interaction of nc886, a cellular non-coding RNA, and PKR. FEBS Letters, 586(19), 3477-3484. https://doi.org/10.1016/j.febslet.2012.07.076