TY - JOUR
T1 - Characterization of the gamboa virus serogroup (orthobunyavirus genus, peribunyaviridae family)
AU - Chiang, Jannifer Oliveira
AU - de Souza, William Marciel
AU - Nunes, Márcio Roberto Teixeira
AU - Acrani, Gustavo Olszanski
AU - Travassos da Rosa, Amélia Paes de Andrade
AU - de Freitas, Nelma Mesquita
AU - da Silva, Sandro Patroca
AU - da Silva, Pedro Henrique Dorta
AU - de Sousa, Alana Watanabe
AU - Rodrigues, Sueli Guerreiro
AU - Quaresma, Juarez Antônio Simões
AU - Dutary, Bedsy
AU - Guzmán, Hilda
AU - Vasilakis, Nikos
AU - Tesh, Robert B.
AU - Vasconcelos, Pedro Fernando da Costa
N1 - Publisher Copyright:
Copyright © 2018 by The American Society of Tropical Medicine and Hygiene.
PY - 2018
Y1 - 2018
N2 - Comprehensive comparative phylogenetic analyses were performed on 17 Gamboa serogroup viruses (GAMSVs) from distinct geographic regions in the Americas and other representative members of the genus Orthobunyavirus (Peribunyaviridae), based on small (S), medium (M), and large (L) open reading frame full-length and partial sequences. Genome characterization showed that the GAMSVs divide into four clades or genotypes. The GAMSVs have a genetic organization similar to other orthobunyaviruses, except that they have a larger NSm protein than other orthobunyaviruses. A serosurvey for Gamboa virus antibodies was performed in plasma from birds, other wild animals, and humans living around the Tucuruí hydroelectric dam in Pará state, northern Brazil, a known focus of GAMSV activity. Newborn chicks (Gallus gallus domesticus) were experimentally infected with a GAMSV, and the pathogenesis is described. Histopathological changes were primarily in the lungs and liver. Also, a review of the ecology of the GAMSVs in the Americas is included. In sum, this study presents the genomic and evolutionary characterization of the Gamboa group and the potential model of pathogenesis, which would be helpful for diagnostic purposes, epidemiology, and immuno-pathogenesis studies.
AB - Comprehensive comparative phylogenetic analyses were performed on 17 Gamboa serogroup viruses (GAMSVs) from distinct geographic regions in the Americas and other representative members of the genus Orthobunyavirus (Peribunyaviridae), based on small (S), medium (M), and large (L) open reading frame full-length and partial sequences. Genome characterization showed that the GAMSVs divide into four clades or genotypes. The GAMSVs have a genetic organization similar to other orthobunyaviruses, except that they have a larger NSm protein than other orthobunyaviruses. A serosurvey for Gamboa virus antibodies was performed in plasma from birds, other wild animals, and humans living around the Tucuruí hydroelectric dam in Pará state, northern Brazil, a known focus of GAMSV activity. Newborn chicks (Gallus gallus domesticus) were experimentally infected with a GAMSV, and the pathogenesis is described. Histopathological changes were primarily in the lungs and liver. Also, a review of the ecology of the GAMSVs in the Americas is included. In sum, this study presents the genomic and evolutionary characterization of the Gamboa group and the potential model of pathogenesis, which would be helpful for diagnostic purposes, epidemiology, and immuno-pathogenesis studies.
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U2 - 10.4269/ajtmh.17-0810
DO - 10.4269/ajtmh.17-0810
M3 - Article
C2 - 29436340
AN - SCOPUS:85046888573
SN - 0002-9637
VL - 98
SP - 1502
EP - 1511
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 5
ER -