Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy

Lawrence C. Sowers, Ramon Eritja, Fu Ming Chen, Tasneem Khwaja, Bruce E. Kaplan, Myron F. Goodman, G. Victor Fazakerley

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Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP·C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP·C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.

Original languageEnglish (US)
Pages (from-to)89-92
Number of pages4
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Nov 30 1989
Externally publishedYes


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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