Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy

Lawrence Sowers, Ramon Eritja, Fu Ming Chen, Tasneem Khwaja, Bruce E. Kaplan, Myron F. Goodman, G. Victor Fazakerley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP·C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP·C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.

Original languageEnglish (US)
Pages (from-to)89-92
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume165
Issue number1
DOIs
StatePublished - Nov 30 1989
Externally publishedYes

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Oligonucleotides
Base Pairing
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
2-Aminopurine
DNA
Nuclear magnetic resonance
DNA Replication
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy. / Sowers, Lawrence; Eritja, Ramon; Chen, Fu Ming; Khwaja, Tasneem; Kaplan, Bruce E.; Goodman, Myron F.; Victor Fazakerley, G.

In: Biochemical and Biophysical Research Communications, Vol. 165, No. 1, 30.11.1989, p. 89-92.

Research output: Contribution to journalArticle

Sowers, Lawrence ; Eritja, Ramon ; Chen, Fu Ming ; Khwaja, Tasneem ; Kaplan, Bruce E. ; Goodman, Myron F. ; Victor Fazakerley, G. / Characterization of the high pH wobble structure of the 2-aminopurine·cytosine mismatch by N-15 NMR spectroscopy. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 165, No. 1. pp. 89-92.
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