Characterization of the immunosuppressive effect of burned tissue in an animal model

John F. Hansbrough, Ramon Zapata Sirvent, V. Peterson, X. Wang, Edward Bender, Henry Claman, John Boswick

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Abstract

The immunosuppressive effect of burned tissue was studied using a mouse burn model. To evaluate the immunologic status an in vivo measure of cell-mediated immunity (CMI) involving contact sensitization of mice by painting the skin with dinitrofluorobenzene was used; mice were challenged 5 days later by painting the ear with the same antigen. Ear swelling in response to antigenic challenge was used as a quantitative measure of CMI; diminution in ear swelling in treatment mice compared to sensitized, unburned control mice indicated the degree of immunosuppression. A full-thickness steam burn covering 20% body surface ares (BSA) was profoundly immunosuppressive as reflected by ear swelling of 45 to 60% of that found in normal mice; partial thickness burns and burns of 10% BSA extent were not significantly immunosuppressive. Transfer into unburned mice of burned skin equivalent in size to a 20% BSA burn eschar resulted in marked immunosuppression, but transfer of smaller amounts of burned skin, or of larger amounts of unburned skin and normal and burned liver tissue, did not produce immunosuppression. Mice receiving a very high-temperature (300°C), dry burn were only slightly more suppressed than mice receiving a standard steam burn. Normal immunity was preserved in burned mice which received daily application of cerium nitrate to the wound for 7 days, but application of other topical agents commonly used in burn treatment did not preserve immunity. Postburn immunosuppression thus appears related quantitatively to toxic factors in burned skin, and these toxic factors can be abrogated in burned mice by the topical application of cerium nitrate.

Original languageEnglish (US)
Pages (from-to)383-393
Number of pages11
JournalJournal of Surgical Research
Volume37
Issue number5
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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Immunosuppressive Agents
Animal Models
Immunosuppression
Ear
Skin
Paintings
Poisons
Steam
Burns
Cellular Immunity
Immunity
Dinitrofluorobenzene
Antigens
Temperature

ASJC Scopus subject areas

  • Surgery

Cite this

Characterization of the immunosuppressive effect of burned tissue in an animal model. / Hansbrough, John F.; Zapata Sirvent, Ramon; Peterson, V.; Wang, X.; Bender, Edward; Claman, Henry; Boswick, John.

In: Journal of Surgical Research, Vol. 37, No. 5, 01.01.1984, p. 383-393.

Research output: Contribution to journalArticle

Hansbrough, John F. ; Zapata Sirvent, Ramon ; Peterson, V. ; Wang, X. ; Bender, Edward ; Claman, Henry ; Boswick, John. / Characterization of the immunosuppressive effect of burned tissue in an animal model. In: Journal of Surgical Research. 1984 ; Vol. 37, No. 5. pp. 383-393.
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