Characterization of the inflammatory response during acute and post-acute phases after severe burn

Gerd G. Gauglitz, Juquan Song, David Herndon, Celeste Finnerty, Darren Boehning, José M. Barral, Marc G. Jeschke

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Severe burn causes a pronounced hypermetabolic response characterized by catabolism and extensive protein wasting. We recently found that this hypermetabolic state is driven by a severe inflammatory response. We characterized in detail the kinetics of serum levels of a panel of cytokines in a rat model, which may serve as reference for the development of therapeutic interventions applicable to humans. Male Sprague-Dawley rats (n = 8) received a full-thickness burn of 60% total body surface area. Serum was harvested 1, 3, 6, 12, 24, 48, 96, and 168 h after burn. Eight serum cytokines commonly used to assess the inflammatory response in humans, such as IL-1β, IL-6, IL-10, TNF, vascular endothelial growth factor, and monocyte chemotactic protein 1, and the rat-specific cytokines cytokine-induced neutrophil chemoattractant (CINC) 1, CINC-2, and CINC-3 were measured by enzyme-linked immunosorbent assay technique and were compared with controls (n = 4). Statistical analysis was conducted using the t test, with P < 0.05 considered as significantly different. Thermal injury resulted in significantly increased serum levels of IL-1β, IL-6, IL-10, monocyte chemotactic protein 1, CINC-1, CINC-2, and CINC-3 when compared with the concentrations detected in nonburned rats (P < 0.05). Serum levels of TNF-α and vascular endothelial growth factor in burned rats were not found to be significantly different to controls. Burn causes a profound inflammatory response in rats. Specific cytokines known to increase in humans postburn such as IL-1 β, IL-6, IL-10, MCP-1, and IL-8 (CINC-1, CINC-2, and CINC-3 in the rat) were also observed in our rat burn model, which now allows us to study new anti-inflammatory treatment options.

Original languageEnglish (US)
Pages (from-to)503-507
Number of pages5
JournalShock
Volume30
Issue number5
DOIs
StatePublished - Nov 2008

Fingerprint

Chemotactic Factors
Cytokines
Neutrophils
Interleukin-1
Interleukin-10
Interleukin-6
Serum
Vascular Endothelial Growth Factor A
Immunosorbent Techniques
Chemokine CCL2
Body Surface Area
Interleukin-8
Sprague Dawley Rats
Anti-Inflammatory Agents
Hot Temperature
Enzyme-Linked Immunosorbent Assay
Wounds and Injuries
Therapeutics

Keywords

  • Burn
  • CINC
  • Cytokines
  • IL-6
  • Inflammation

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Characterization of the inflammatory response during acute and post-acute phases after severe burn. / Gauglitz, Gerd G.; Song, Juquan; Herndon, David; Finnerty, Celeste; Boehning, Darren; Barral, José M.; Jeschke, Marc G.

In: Shock, Vol. 30, No. 5, 11.2008, p. 503-507.

Research output: Contribution to journalArticle

Gauglitz, Gerd G. ; Song, Juquan ; Herndon, David ; Finnerty, Celeste ; Boehning, Darren ; Barral, José M. ; Jeschke, Marc G. / Characterization of the inflammatory response during acute and post-acute phases after severe burn. In: Shock. 2008 ; Vol. 30, No. 5. pp. 503-507.
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