Characterization of the outer membrane protein profile from disease-related Helicobacter pylori isolates by subcellular fractionation and nano-LC FT-ICR MS analysis

Elisabet Carlsohn, Johanna Nyström, Hasse Karlsson, Ann Mari Svennerholm, Carol L. Nilsson

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Because of the important role of membrane proteins in adhesion, invasion, and intracellular survival of pathogens in the host, membrane proteins are of potential interest in the search for drug targets or biomarkers. We have established a mass spectrometry-based method that allows characterization of the outer membrane protein (OMP) profile of clinical isolates from of the human gastric pathogen Helicobacter pylori. Subcellular fractionation and one-dimensional gel electrophoresis (1 D-GE) analysis was combined with nano-liquid chromatography Fourier transform-ion cyclotron resonance mass spectrometry (nano-LC FT-ICR MS) and tandem mass spectrometry (MS/MS) analysis of fifteen H. pylori strains associated either with duodenal ulcers, gastric cancer, or isolated from asymptomatic H. pylori infected carriers. Over 60 unique membrane or membrane-associated proteins, including 30 of the 33 theoretically predicted OMPs, were identified from the strains. Several membrane proteins, including Omp11 and BabA, were found to be expressed by all strains. In the search for clinical markers we found that Omp26 was expressed by all disease-related strains but was only present in one out of five strains from asymptomatic carriers, which makes Omp26 a potential target for further investigation in the search for proteins unique to disease-related H. pylori strains. In addition, presence of Omp30 and absence of Omp6 seemed to be associated with H. pylori strains causing duodenal ulcer.

Original languageEnglish (US)
Pages (from-to)3197-3204
Number of pages8
JournalJournal of Proteome Research
Volume5
Issue number11
DOIs
StatePublished - Nov 2006
Externally publishedYes

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Fractionation
Helicobacter pylori
Membrane Proteins
Mass spectrometry
Duodenal Ulcer
Pathogens
Mass Spectrometry
Biomarkers
Cyclotrons
Fourier Analysis
Cyclotron resonance
Tandem Mass Spectrometry
Liquid Chromatography
Membrane Potentials
Liquid chromatography
Stomach Neoplasms
Electrophoresis
Stomach
Gels
Ions

Keywords

  • Biomarkers
  • FT-ICR MS
  • H. pylori
  • Nano-LC
  • OMP
  • Outer membrane
  • Vaccine antigen

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Characterization of the outer membrane protein profile from disease-related Helicobacter pylori isolates by subcellular fractionation and nano-LC FT-ICR MS analysis. / Carlsohn, Elisabet; Nyström, Johanna; Karlsson, Hasse; Svennerholm, Ann Mari; Nilsson, Carol L.

In: Journal of Proteome Research, Vol. 5, No. 11, 11.2006, p. 3197-3204.

Research output: Contribution to journalArticle

Carlsohn, Elisabet ; Nyström, Johanna ; Karlsson, Hasse ; Svennerholm, Ann Mari ; Nilsson, Carol L. / Characterization of the outer membrane protein profile from disease-related Helicobacter pylori isolates by subcellular fractionation and nano-LC FT-ICR MS analysis. In: Journal of Proteome Research. 2006 ; Vol. 5, No. 11. pp. 3197-3204.
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