Characterization of the second long polar (LP) fimbriae of Escherichia coli O157

H7 and distribution of LP fimbriae in other pathogenic E. coli strains

Alfredo Torres, Kristen J. Kanack, Christopher B. Tutt, Vsevolod Popov, James B. Kaper

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

A second region containing five genes homologous to the long polar fimbrial operon of Salmonella enterica serovar Typhimurium is located in the chromosome of enterohemorrhagic Escherichia coli (EHEC) O157:H7. A non-fimbriated E. coli K-12 strain carrying the cloned EHEC lpf (lpf2) genes expressed thin fibrillae-like structures on its surface and displayed reduced adherence to tissue culture cells. Neither mutation in the lpfA2 gene in either the parent or lpfA1 mutant strains showed an effect in adherence or in the formation of A/E lesions on HeLa cells. lpfA2 isogenic mutant strains adhere to Caco-2 cells almost as well as the wild-type at 5 h, but they were deficient in adherence at early time points. A collection of diarrheagenic E. coli strains were investigated for the presence of lpfA1 and lpfA2 and results showed that these genes are present in specific serogroups which are phylogenetically related. Our results suggest that LP fimbriae 2 may contribute to the early stages of EHEC adhesion and that genes encoding the major LP fimbrial subunits are present in a small group of EHEC and EPEC serotypes.

Original languageEnglish (US)
Pages (from-to)333-344
Number of pages12
JournalFEMS Microbiology Letters
Volume238
Issue number2
DOIs
StatePublished - Sep 15 2004

Fingerprint

Escherichia coli O157
Enterohemorrhagic Escherichia coli
Escherichia coli
Genes
Enteropathogenic Escherichia coli
Salmonella enterica
Caco-2 Cells
Operon
HeLa Cells
Cell Culture Techniques
Chromosomes
Mutation
Serogroup

Keywords

  • Enterohemorrhagic Escherchia coli
  • FAS assay
  • Long polar fimbriae

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Characterization of the second long polar (LP) fimbriae of Escherichia coli O157 : H7 and distribution of LP fimbriae in other pathogenic E. coli strains. / Torres, Alfredo; Kanack, Kristen J.; Tutt, Christopher B.; Popov, Vsevolod; Kaper, James B.

In: FEMS Microbiology Letters, Vol. 238, No. 2, 15.09.2004, p. 333-344.

Research output: Contribution to journalArticle

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